Engineering Research Center of the Ministry of Education, Jilin Agricultural University, Changchun 130118, China; College of traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China.
School of Food and Pharmaceutical Engineering, Wuzhou University, Wuzhou 543002, China.
Int J Biol Macromol. 2023 Sep 30;249:126014. doi: 10.1016/j.ijbiomac.2023.126014. Epub 2023 Jul 29.
Inadequate angiogenesis and inflammation at the wound site have always been a major threat to skin wounds, especially for diabetic wounds that are difficult to heal. Therefore, hydrogel dressings with angiogenesis and antibacterial properties are very necessary in practical applications. This study reported a hydrogel (PCA) based on L-arginine conjugated chitosan (CA) and aldehyde functionalized polysaccharides of Phellinus igniarius (OPPI) as an antibacterial and pro-angiogenesis dressing for wound repair in diabetes for the first time. and discussed its possible mechanism for promoting wound healing. The results showed that PCA had good antioxidant, antibacterial, biological safety and other characteristics, and effectively promoted the healing course of diabetic wound model. In detail, the H&E and Masson staining results showed that PCA promoted normal epithelial formation and collagen deposition. The Western blot results confirmed that PCA decreased the inflammation by inhibiting the IKBα/NF-κB signaling pathway and enhanced angiogenesis by adjusting the level of HIF-1α. In conclusion, PCA is a promising candidate for promoting wound healing in diabetes. Graphic abstract.
在伤口部位，血管生成和炎症不足一直是皮肤伤口的主要威胁，特别是对于难以愈合的糖尿病伤口。因此，在实际应用中，具有血管生成和抗菌性能的水凝胶敷料非常必要。本研究首次报道了一种基于 L-精氨酸接枝壳聚糖（CA）和香菇多糖醛基功能化多糖（OPPI）的水凝胶（PCA），作为一种用于糖尿病伤口修复的具有抗菌和促血管生成作用的敷料，并探讨了其促进伤口愈合的可能机制。结果表明，PCA 具有良好的抗氧化、抗菌、生物安全性等特性，能有效促进糖尿病伤口模型的愈合过程。具体而言，H&E 和 Masson 染色结果表明，PCA 通过抑制 IKBα/NF-κB 信号通路促进正常上皮形成和胶原沉积，Western blot 结果证实 PCA 通过调节 HIF-1α 水平抑制炎症，增强血管生成。总之，PCA 是一种有前途的促进糖尿病伤口愈合的候选药物。
