Mai Le Thi Phuong, Tran Van Dinh, Phuong Hoang Vu Mai, Trang Ung Thi Hong, Thanh Le Thi, Son Nguyen Vu, Cuong Vuong Duc, Dung Luu Phuong, Hanh Nguyen Thi My, Hai Hoang, Oanh Dang Thi Kieu, Thuy Nguyen Thanh
Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam.
Environ Chall (Amst). 2022 Apr;7:100526. doi: 10.1016/j.envc.2022.100526. Epub 2022 Apr 22.
The objective of the paper is to investigate the presence of SARS-CoV-2 on inanimate surfaces in four healthcare facilities treating patients with COVID-19 and four quarantine regiments of provincial military commands.
From August to October 2020, a total of 468 one-off environmental samples consisting of inanimate surfaces, garbage, and wastewater were collected. The real-time RT-PCR assay targeting E and RdRp genes to detect SARS-CoV-2 and checklist and questionnaire of disinfection practices were employed. If detected by RT-PCR, then positive samples are subjected to cell culture to determine viability.
The test results showed all samples (100%) to be negative with SARS-CoV-2 resulting in unperformed virus culture. As for recent disinfection practices, chlorine-based products dissolved at a concentration of 0.1% (1000 ppm) in the general context or 0.5% (5000 ppm) for blood and body fluid spills are routinely applied twice a day and at the discharge of patients or quarantined people.
The finding may illustrate the importance of disinfection practices in removing pathogens or significantly reducing SARS-CoV-2 contamination on environmental surfaces and waste.
本文旨在调查四家收治新冠肺炎患者的医疗机构以及省军区四个隔离团的无生命表面上是否存在严重急性呼吸综合征冠状病毒2（SARS-CoV-2）。
2020年8月至10月，共采集了468份一次性环境样本，包括无生命表面、垃圾和废水。采用针对E基因和RdRp基因的实时逆转录聚合酶链反应（RT-PCR）检测法来检测SARS-CoV-2，并使用了消毒操作清单和问卷。若RT-PCR检测呈阳性，则对阳性样本进行细胞培养以确定病毒活性。
检测结果显示，所有样本（100%）的SARS-CoV-2检测均为阴性，因此未进行病毒培养。至于近期的消毒操作，通常在一般情况下使用浓度为0.1%（1000 ppm）的含氯产品，对于血液和体液溢出物则使用0.5%（5000 ppm）的含氯产品，每天常规使用两次，在患者或被隔离人员出院时也会使用。
这一发现可能说明了消毒操作在清除病原体或显著减少环境表面和废物上的SARS-CoV-2污染方面的重要性。
