在伊朗西南部胡齐斯坦省使用高分辨率熔解分析（HRM分析）对艾滋病患者进行基因分型。
genotyping in AIDS patients using high resolution melt analysis (HRM analysis) in Khuzestan province, southwest Iran.
作者信息
Saki J, Arjmand Reza
机构信息
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Faculty of Medicine, Department of Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
J Parasit Dis. 2023 Sep;47(3):630-634. doi: 10.1007/s12639-023-01601-x. Epub 2023 Jun 14.
Toxoplasmosis is a parasitic zoonotic disease caused by , a common protozoan in the Apicomplexa phylum. Several studies in Iran have demonstrated the presence of the parasite in various hosts, but no data on genotyping in HIV patients in Khuzestan, Southwest Iran, is available. One hundred of blood samples from AIDS patients were collected and tested by real-time PCR High Resolution Melting analyses for detection and genotyping. was discovered in 8 out of 100 (8%) AIDS patients with dominant Type I. This study suggest that HRM method demonstrated excellent discriminating ability for , and AIDS patients should be tested for detection and genotyping to prevent parasite pathogenicity.
弓形虫病是一种由刚地弓形虫引起的寄生性人畜共患病，刚地弓形虫是顶复门中一种常见的原生动物。伊朗的多项研究表明该寄生虫在各种宿主中均有存在，但伊朗西南部胡齐斯坦省艾滋病毒患者的弓形虫基因分型数据尚无可用信息。收集了100例艾滋病患者的血液样本，并通过实时聚合酶链反应高分辨率熔解分析进行检测和基因分型，以检测弓形虫。在100例艾滋病患者中有8例（8%）检测出弓形虫，且以I型为主。本研究表明，高分辨率熔解方法对弓形虫具有出色的鉴别能力，应对艾滋病患者进行弓形虫检测和基因分型，以预防寄生虫致病性。
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