亚麻籽提取物调节高脂饮食喂养的去卵巢大鼠治疗模型中的生化和组织形态学变化。

L. seed extract modulates biochemical and histomorphological changes in therapeutic model of high-fat diet-fed ovariectomized rats.

作者信息

Nagamma Takkella, Konuri Anjaneyulu, Bhat Kumar M R, Udupa Padmanabha E G, Nayak Yogendra

机构信息

Department of Biochemistry, Manipal TATA Medical College, Manipal Academy of Higher Education, Manipal, Karnataka 576104 India.

Department of Anatomy, Manipal TATA Medical College, Manipal Academy of Higher Education, Manipal, Karnataka 576104 India.

出版信息

3 Biotech. 2023 Aug;13(8):285. doi: 10.1007/s13205-023-03707-8. Epub 2023 Jul 28.

DOI:10.1007/s13205-023-03707-8

PMID:37520342

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10382425/

Abstract

UNLABELLED

This study investigates the therapeutic effect of petroleum ether fraction of L. (PE-TFG) seed extract in ovariectomized rats fed with high-fat diet. Rats were randomly grouped into sham ovariectomy (S.OVX), ovariectomy + high-fat diet (OVX + HFD), and treatment groups. The blood samples were collected, and lipid profile, glucose, hepatic markers, and inflammatory markers were estimated. Liver, kidney, and common carotid artery were isolated for histopathological observations. Liver samples were tested for antioxidant, oxidative stress markers, mRNA expression of adiponectin, and PPAR-γ. PE-TFG treatment significantly decreased total cholesterol (18%), LDL (20%), hepatic markers (28%), leptin (17%), TNF-α (21%), and increased mRNA expression of adiponectin and PPAR-γ. There was also micro- and macro-hepatic steatosis, inflammation in the liver, deteriorated tubules in the kidney, and increased tunica intima and media thickness of the common carotid artery. These pathological alterations were reversed with PE-TFG administration. This impact might be linked to phytoestrogens and other components in PE-TFG such as diosgenin, phenols, and flavonoids.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-023-03707-8.

摘要

未标注

本研究调查了光叶巴豆种子提取物的石油醚馏分（PE-TFG）对高脂饮食喂养的去卵巢大鼠的治疗效果。将大鼠随机分为假去卵巢组（S.OVX）、去卵巢+高脂饮食组（OVX+HFD）和治疗组。采集血样，检测血脂、血糖、肝脏标志物和炎症标志物。分离肝脏、肾脏和颈总动脉进行组织病理学观察。检测肝脏样本的抗氧化剂、氧化应激标志物、脂联素和PPAR-γ的mRNA表达。PE-TFG治疗显著降低了总胆固醇（18%）、低密度脂蛋白（20%）、肝脏标志物（28%）、瘦素（17%）、肿瘤坏死因子-α（21%），并增加了脂联素和PPAR-γ的mRNA表达。还存在肝脏微脂肪变性和大脂肪变性、肝脏炎症、肾脏肾小管恶化以及颈总动脉内膜和中膜厚度增加。PE-TFG给药可逆转这些病理改变。这种影响可能与PE-TFG中的植物雌激素和其他成分如薯蓣皂苷元、酚类和黄酮类有关。