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与粉蠹虫相关的某物种中，与Bqt4同源的FspTF转录因子的生物学相关性。

The biological relevance of the FspTF transcription factor, homologous of Bqt4, in sp. associated with the ambrosia beetle .

作者信息

Carreras-Villaseñor Nohemí, Martínez-Rodríguez Luis A, Ibarra-Laclette Enrique, Monribot-Villanueva Juan L, Rodríguez-Haas Benjamín, Guerrero-Analco José A, Sánchez-Rangel Diana

机构信息

Laboratorios de Biología Molecular y Fitopatología, Instituto de Ecología A.C. (INECOL), Red de Estudios Moleculares Avanzados (REMAv), Xalapa, Mexico.

Laboratorio de Genómica y Transcriptómica, Instituto de Ecología A.C. (INECOL), Red de Estudios Moleculares Avanzados (REMAv), Xalapa, Mexico.

出版信息

Front Microbiol. 2023 Jul 14;14:1224096. doi: 10.3389/fmicb.2023.1224096. eCollection 2023.

DOI:10.3389/fmicb.2023.1224096
PMID:37520351
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10375492/
Abstract

Transcription factors in phytopathogenic fungi are key players due to their gene expression regulation leading to fungal growth and pathogenicity. The KilA-N family encompasses transcription factors unique to fungi, and the Bqt4 subfamily is included in it and is poorly understood in filamentous fungi. In this study, we evaluated the role in growth and pathogenesis of the homologous of Bqt4, FspTF, in sp. isolated from the ambrosia beetle through the characterization of a CRISPR/Cas9 edited strain in Fsp The phenotypic analysis revealed that TF65-6, the edited strain, modified its mycelia growth and conidia production, exhibited affectation in mycelia and culture pigmentation, and in the response to certain stress conditions. In addition, the plant infection process was compromised. Untargeted metabolomic and transcriptomic analysis, clearly showed that FspTF may regulate secondary metabolism, transmembrane transport, virulence, and diverse metabolic pathways such as lipid metabolism, and signal transduction. These data highlight for the first time the biological relevance of an orthologue of Bqt4 in sp. associated with an ambrosia beetle.

摘要

植物病原真菌中的转录因子是关键角色，因为它们调控基因表达，从而影响真菌的生长和致病性。KilA-N家族包含真菌特有的转录因子，Bqt4亚家族属于该家族，在丝状真菌中人们对其了解较少。在本研究中，我们通过对尖孢镰刀菌（Fsp）中CRISPR/Cas9编辑菌株的特性分析，评估了Bqt4同源物FspTF在从 ambrosia beetle分离出的尖孢镰刀菌中的生长和致病作用。表型分析表明，编辑菌株TF65-6改变了其菌丝生长和分生孢子产生，在菌丝体和培养物色素沉着以及对某些胁迫条件的反应方面表现出影响。此外，植物感染过程也受到损害。非靶向代谢组学和转录组学分析清楚地表明，FspTF可能调节次级代谢、跨膜运输、毒力以及多种代谢途径，如脂质代谢和信号转导。这些数据首次突出了与ambrosia beetle相关的尖孢镰刀菌中Bqt4直系同源物的生物学相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/dfd09068b958/fmicb-14-1224096-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/544c9713eccb/fmicb-14-1224096-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/abb9f1145edb/fmicb-14-1224096-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/5fe4fd224ac4/fmicb-14-1224096-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/757c561e2f45/fmicb-14-1224096-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/aefacdd74456/fmicb-14-1224096-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/69f875c38d4a/fmicb-14-1224096-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/3e207aafa4b9/fmicb-14-1224096-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/055e95c0806f/fmicb-14-1224096-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/dfd09068b958/fmicb-14-1224096-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/544c9713eccb/fmicb-14-1224096-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/abb9f1145edb/fmicb-14-1224096-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/5fe4fd224ac4/fmicb-14-1224096-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/757c561e2f45/fmicb-14-1224096-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/aefacdd74456/fmicb-14-1224096-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/69f875c38d4a/fmicb-14-1224096-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/3e207aafa4b9/fmicb-14-1224096-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/055e95c0806f/fmicb-14-1224096-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09bf/10375492/dfd09068b958/fmicb-14-1224096-g009.jpg

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