Calleros-González Paulette, Ibarra-Juarez Arturo, Lamelas Araceli, Suárez-Moo Pablo
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Xalapa, C.P. 91070, México.
Investigador Por México - CONAHCyT. Instituto de Ecología, A. C., Carretera Antigua a Coatepec 351, Xalapa, C.P. 91070, México.
Int Microbiol. 2024 Dec;27(6):1641-1654. doi: 10.1007/s10123-024-00502-0. Epub 2024 Mar 15.
The ambrosia beetles are farming insects that feed mainly on their cultivated fungi, which in some occasions are pathogens from forest and fruit trees. We used a culture-independent approach based on 16S and 18S rRNA gene metabarcoding analysis to investigate the diversity and composition of the bacterial and fungal communities associated with five ambrosia beetle species: four species native to America (Monarthrum dimidiatum, Dryocoetoides capucinus, Euwallacea discretus, Corthylus consimilis) and an introduced species (Xylosandrus morigerus). For the bacterial community, the beetle species hosted a broad diversity with 1,579 amplicon sequence variants (ASVs) and 66 genera, while for the fungal community they hosted 288 ASVs and 39 genera. Some microbial groups dominated the community within a host species or a body part (Wolbachia in the head-thorax of E. discretus; Ambrosiella in the head-thorax and abdomen of X. morigerus). The taxonomic composition and structure of the microbial communities appeared to differ between beetle species; this was supported by beta-diversity analysis, which indicated that bacterial and fungal communities were clustered mainly by host species. This study characterizes for the first time the microbial communities associated with unexplored ambrosia beetle species, as well as the factors that affect the composition and taxonomic diversity per se, contributing to the knowledge of the ambrosia beetle system.
食菌小蠹是一类营共生生活的昆虫，主要以它们培育的真菌为食，这些真菌在某些情况下是森林和果树上的病原体。我们采用了一种基于16S和18S rRNA基因宏条形码分析的非培养方法，来研究与五种食菌小蠹相关的细菌和真菌群落的多样性和组成：四种原产于美洲的物种（半黑额小蠹、帽状材小蠹、离散材小蠹、相似材小蠹）和一种引入物种（褐粉小蠹）。对于细菌群落，这些小蠹物种拥有广泛的多样性，有1579个扩增子序列变体（ASV）和66个属，而对于真菌群落，它们拥有288个ASV和39个属。一些微生物类群在宿主物种或身体部位内主导着群落（离散材小蠹头胸部的沃尔巴克氏体；褐粉小蠹头胸部和腹部的长喙壳菌）。微生物群落的分类组成和结构在不同小蠹物种之间似乎存在差异；这得到了β多样性分析的支持，该分析表明细菌和真菌群落主要按宿主物种聚类。本研究首次对与未被探索的食菌小蠹物种相关的微生物群落，以及影响其组成和分类多样性本身的因素进行了表征，有助于增进对食菌小蠹系统的了解。
