Host Microbe Interaction Research Laboratory, Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Japan.
Laboratory of Food Immunology, Department of Science and Technology on Food Safety, Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Japan.
Front Cell Infect Microbiol. 2023 Jul 13;13:1056866. doi: 10.3389/fcimb.2023.1056866. eCollection 2023.
In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by analysis, and it is necessary to study the intestinal bacteria by culturing them. In addition, various media recommended for each individual bacterium have been used for culturing intestinal bacteria; however, the preparation of each medium is complex. To simultaneously culture many bacteria and compare bacterial phenotypes under the same conditions, a medium capable of culturing a wide range of bacteria is needed. In this study, we developed GAM + blood medium (GB medium), which consists of Gifu anaerobic medium containing 5% (v/v) horse blood; it is easy to prepare and it allowed the successful cultivation of 85% of the available predominant species in the human intestinal microbiota.
近年来，随着非培养方法的发展，肠道细菌对人类健康的影响已变得明显。然而，由于三分之一的基因无法被注释，通过分析来阐明所有肠道细菌的功能变得困难，因此有必要通过培养它们来研究肠道细菌。此外，已经使用推荐用于每种单个细菌的各种培养基来培养肠道细菌；但是，每种培养基的制备都很复杂。为了同时培养多种细菌并在相同条件下比较细菌表型，需要一种能够培养广泛细菌的培养基。在这项研究中，我们开发了 GAM + 血培养基（GB 培养基），它由含有 5%（v/v）马血的吉富厌氧培养基组成；它易于制备，并且能够成功培养人类肠道微生物群中 85%的现有主要物种。
