Lu Lili, Tang Yuhan, Xu Huajie, Qian Yi, Tao Jun, Zhao Daqiu
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, 225009 Jiangsu China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, 225009 Jiangsu China.
Physiol Mol Biol Plants. 2023 Jun;29(6):773-782. doi: 10.1007/s12298-023-01325-5. Epub 2023 Jun 19.
Herbaceous peony ( Pall.) has emerged in the cut flower market due to its beautiful appearance. The bending flower stems caused by a lack of mechanical strength is the main problem restricting the development of the cut industry. So it is of great worth to reveal the basis of stem development changes in to improve its cut flower quality. Quantitative research on gene expression characteristics can provide clues for understanding their biological functions, and the screening of relatively stable expression genes is a prerequisite for the quantitative study of gene expression characteristics. Thus, it is necessary to find appropriate genes during stem development so as to analyze the qRT‒PCR results. In this study, 10 genes were screened, and these genes expressed stably in stems of different stem strengths at three different developmental stages. Then, their expressions were evaluated by RefFinder, BestKeeper, NormFinder, and GeNorm programs. The results demonstrated that γ-tubulin () was the most suitable gene, followed by α-tubulin () and β-D-glucosidase (), whereas histone H3 () was the least suitable gene. Besides, the temporal and spatial expression characteristics of , the key gene concerned with the synthesis of cell wall fillers in , were also used to evaluate the suitability of genes. Consequently, and are the two best combinations during stem development, and their combination can be used for the stem development of These findings will provide a reference for the selection of genes related to stem development and the study of molecular mechanisms related to stem development in .
The online version contains supplementary material available at 10.1007/s12298-023-01325-5.
芍药（Pall.）因其美丽的外观已出现在切花市场。缺乏机械强度导致花茎弯曲是限制切花产业发展的主要问题。因此，揭示芍药茎发育变化的基础对于提高其切花品质具有重要价值。对基因表达特征进行定量研究可为理解其生物学功能提供线索，筛选相对稳定表达的基因是基因表达特征定量研究的前提。因此，有必要在茎发育过程中找到合适的基因以分析qRT-PCR结果。本研究筛选了10个基因，这些基因在三个不同发育阶段的不同茎强度的茎中稳定表达。然后，通过RefFinder、BestKeeper、NormFinder和GeNorm程序评估它们的表达。结果表明，γ-微管蛋白（）是最合适的基因，其次是α-微管蛋白（）和β-D-葡萄糖苷酶（），而组蛋白H3（）是最不合适的基因。此外，还利用芍药中与细胞壁填充物合成相关的关键基因的时空表达特征来评估基因的适用性。因此，和是茎发育过程中两个最佳组合，它们的组合可用于芍药的茎发育。这些发现将为芍药茎发育相关基因的选择以及芍药茎发育相关分子机制的研究提供参考。
在线版本包含可在10.1007/s12298-023-01325-5获取的补充材料。
