CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Innovation Academy for Seed Design, Chinese Academy of Sciences, Menglun, Mengla 666303, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2021 Mar 4;22(5):2569. doi: 10.3390/ijms22052569.
Tiger nut (), a perennial C plant of the family, is an unconventional crop that is distinguished by its oil-rich tubers, which also possesses the advantages of strong resistance, wide adaptability, short life periods, and large biomass. To facilitate studies on gene expression in this species, we identified and validated a series of reference genes (RGs) based on transcriptome data, which can be employed as internal controls for qRT-PCR analysis in tiger nut. Fourteen putative candidate RGs were identified and evaluated across nine different tissues of two cultivars, and the RGs were analyzed using three different algorithms (geNorm, NormFinder, and BestKeeper). The stability rankings of the candidate RGs were merged into consensus lists with RankAggreg. For the below-ground storage organ of tiger nut, the optimal RGs were and in different developmental stages of tubers. and were the most stably expressed RGs among all tissues, while and exhibited the lowest expression stability. , and were compared to normalize the expression levels of the () and () genes across the same tissues. Our results showed that the RGs identified in this study, which exhibit more uniform expression patterns, may be utilized for the normalization of qRT-PCR results, promoting further research on gene expression in various tissues of tiger nut.
西亚米荠（Tiger nut），是十字花科滨藜属的多年生草本植物，是一种非传统作物，以其富含油脂的块茎而闻名，具有抗性强、适应性广、生育期短、生物量大等优点。为了便于研究该物种的基因表达，我们基于转录组数据鉴定和验证了一系列参考基因（RGs），可作为西亚米荠 qRT-PCR 分析的内参。在两个品种的 9 种不同组织中鉴定和评估了 14 个假定候选 RG，并使用三种不同的算法（geNorm、NormFinder 和 BestKeeper）对 RG 进行了分析。候选 RG 的稳定性排名通过 RankAggreg 合并为共识列表。对于西亚米荠的地下贮藏器官，在块茎的不同发育阶段，最佳 RG 是 和 。在所有组织中， 和 是表达最稳定的 RG，而 和 表现出最低的表达稳定性。与相同组织中的 （）和 （）基因的表达水平进行归一化比较， 、 和 。我们的研究结果表明，本研究中鉴定的 RG 具有更均匀的表达模式，可用于 qRT-PCR 结果的归一化，促进了对西亚米荠不同组织中基因表达的进一步研究。
