Zhou Minghui, Liu Xiang, Zhao Jiayan, Jiang Feng, Li Weitao, Yan Xu, Hu Yonghong, Yuan Junhui
School of Ecological Technology and Engineering, Shanghai Institute of Technology, No. 100 Haiqun Rd., Fengxian District, Shanghai 201416, China.
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, No. 3888 Chenhua Rd., Songjiang District, Shanghai 201602, China.
Plants (Basel). 2024 Nov 27;13(23):3324. doi: 10.3390/plants13233324.
Research on the waterlogging tolerance mechanisms of helps us to further understand these mechanisms in the root system and enhance its root bark and oil yields in southern China. In this study, root morphological identification, the statistics of nine physiological and biochemical indicators, and a comparative transcriptome analysis were used to investigate the waterlogging tolerance mechanism in this plant. As flooding continued, the roots' vigor dramatically declined from 6 to 168 h of waterlogging, the root number was extremely reduced by up to 95%, and the number of roots was not restored after 96 h of recovery. Seven of the nine physiological indicators, including leaf transpiration and photosynthetic rate, stomatal conductance, root activity, and soluble protein and sugar, showed similar trends of gradually declining waterlogging stress and gradual waterlogging recovery, with little difference. However, the leaf conductivity and super oxide dismutase (SOD) activity gradually increased during flooding recovery and decreased in recovery. The tricarboxylic acid (TCA) cycle is essential for plants to grow and survive and plays a central role in the breakdown, or catabolism, of organic fuel molecules, also playing an important biological role in waterlogging stress. In total, 591 potential candidate genes were identified, and 13 particular genes (e.g., isocitrate dehydrogenase (), malate dehydrogenase (), ATP citrate lyase (), succinate dehydrogenase (), and fumarase ()) in the TCA cycle were also tested using qPCR. This study identifies potential candidate genes and provides theoretical support for the breeding, genetic improvement, and enhancement of the root bark yields of , supporting an in-depth understanding of the plant's physiological and molecular response mechanisms to waterlogging stress, helping future research and practice improve plant waterlogging tolerance and promote plant growth and development.
对[植物名称]耐涝机制的研究有助于我们进一步了解其根系中的这些机制，并提高其在中国南方的根皮和油脂产量。在本研究中，通过根系形态鉴定、9种生理生化指标统计以及比较转录组分析来探究该植物的耐涝机制。随着淹水持续，在淹水6至168小时期间根系活力急剧下降，根数量极度减少达95%，且恢复96小时后根数量未恢复。9种生理指标中的7种，包括叶片蒸腾速率、光合速率、气孔导度、根系活力以及可溶性蛋白和糖，在淹水胁迫下呈现逐渐下降以及淹水恢复过程中逐渐恢复的相似趋势，差异不大。然而，叶片电导率和超氧化物歧化酶（SOD）活性在淹水恢复过程中逐渐升高，在恢复后期下降。三羧酸（TCA）循环对植物生长和存活至关重要，在有机燃料分子的分解或分解代谢中起核心作用，在淹水胁迫中也发挥重要生物学作用。总共鉴定出591个潜在候选基因，并且还使用qPCR对TCA循环中的13个特定基因（例如异柠檬酸脱氢酶、苹果酸脱氢酶、ATP柠檬酸裂解酶、琥珀酸脱氢酶和延胡索酸酶）进行了检测。本研究鉴定出潜在候选基因，为[植物名称]的育种、遗传改良和提高根皮产量提供理论支持，有助于深入了解植物对淹水胁迫的生理和分子响应机制，助力未来研究和实践提高植物耐涝性并促进植物生长发育。
