基于掺杂在改性纤维素聚合物薄膜中的铁-没食子酸络合物的新型传感器用于测定乳腺癌患者血清中的CA 15-3 。

Novel sensor for the determination of CA 15-3 in serum of breast cancer patients based on Fe-gallic acid complex doped in modified cellulose polymer thin films.

作者信息

AlGhamdi Hind A, AlZahrani Yasmeen M, Alharthi Salha, Mohy-Eldin Mohamed S, Mohamed Ekram H, Mahmoud Safwat A, Attia Mohamed S

机构信息

Chemistry Department, College of Science, Imam Abdulrahman Bin Faisal University P.O. Box 1982 Dammam 31441 Saudi Arabia.

Polymer Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientifc Research and Technological Applications (SRTA-City) New Borg El-Arab City, P. O. Box: 21934 Alexandria Egypt.

出版信息

RSC Adv. 2023 Jul 28;13(31):21769-21780. doi: 10.1039/d3ra02495d. eCollection 2023 Jul 12.

Fe-gallic acid MOF embedded in an epoxy methyl cellulose polymer (CMC) thin film was synthesized and characterized by different micro-analytical tools such as: FE-SEM/EDX, XPS analysis, XRD analysis, FT-IR, and fluorescence spectroscopy. Fe-gallic acid MOF doped in a stable CMC polymer thin film is used as a novel sensor to identify CA 15-3 in the sera of patients suffering breast malignancy. The presence of appropriate functional groups in aqueous CA 15-3 solutions enables it to interact with the Fe-gallic acid MOF embedded in the thin film. The Fe-gallic acid MOF was found to absorb energy at 350 nm () and emits radiation at 439 nm which was specifically quenched in the presence of CA 15-3 over a working concentration range of 0.05-570 U mL. In contrast to other CA 15-3 detection methods which suffered from electronic noise, interference and slowness, the Fe-gallic acid MOF proved its sensitivity as an economic, stable and reliable probe for the detection and determination of CA 15-3 in patients' serum samples with a detection limit of 0.01 U mL at pH 7.2.

合成了嵌入环氧甲基纤维素聚合物（CMC）薄膜中的铁-没食子酸金属有机框架（MOF），并通过不同的微观分析工具进行了表征，如场发射扫描电子显微镜/能谱仪（FE-SEM/EDX）、X射线光电子能谱分析（XPS分析）、X射线衍射分析（XRD分析）、傅里叶变换红外光谱（FT-IR）和荧光光谱。掺杂在稳定的CMC聚合物薄膜中的铁-没食子酸MOF被用作一种新型传感器，用于识别患有乳腺恶性肿瘤患者血清中的CA 15-3。水性CA 15-3溶液中适当官能团的存在使其能够与嵌入薄膜中的铁-没食子酸MOF相互作用。发现铁-没食子酸MOF在350 nm处吸收能量，并在439 nm处发射辐射，在0.05 - 570 U/mL的工作浓度范围内，在CA 15-3存在时该辐射会被特异性淬灭。与其他存在电子噪声、干扰和检测速度慢等问题的CA 15-3检测方法相比，铁-没食子酸MOF证明了其作为一种经济、稳定且可靠的探针用于检测和测定患者血清样本中CA 15-3的灵敏度，在pH 7.2时检测限为0.01 U/mL。