Anderson K C, Lew M A, Gorgone B C, Martel J, Leamy C B, Sullivan B
Am J Med. 1986 Sep;81(3):405-11. doi: 10.1016/0002-9343(86)90290-1.
The antecubital fossa venipuncture site in frequent blood donors can become scarred or "dimpled" and colonized with surface and deep bacteria that are not sterilized by standard iodophor preparation techniques. These microorganisms can be introduced into blood or platelets at the time of donation. Recent advances that permit the prolonged storage of platelets at room temperature before transfusion allow proliferation of these contaminating bacteria, particularly gram-positive microorganisms. Documented are three episodes of platelet contamination with gram-positive organisms and four cases of sepsis in recipients of these platelets, which were obtained through the "dimpled" site of a single donor and stored for at least 80 hours at 22 degrees C before transfusion. In contrast, platelets harvested from this donor's "dimpled" site and stored for not more than 50 hours were transfused to 25 patients without complication. Therefore, the extended storage of platelets at 22 degrees C prior to transfusion demands strict awareness of any possible sources of extrinsic contamination, since gram-positive bacteria can proliferate under these conditions and result in infectious complications in recipients.
频繁献血者的肘前窝静脉穿刺部位可能会留下疤痕或出现“凹陷”，并被表层和深层细菌定植，而标准碘伏制备技术无法对这些细菌进行消毒。在献血时，这些微生物可能会被带入血液或血小板中。最近的进展使得血小板在输血前能够在室温下长时间储存，这使得这些污染细菌，尤其是革兰氏阳性微生物得以增殖。记录了三起血小板被革兰氏阳性菌污染的事件以及四名接受这些血小板的受血者发生败血症的病例，这些血小板是通过一名献血者的“凹陷”部位采集的，在输血前于22摄氏度下储存了至少80小时。相比之下，从该献血者“凹陷”部位采集并储存不超过50小时的血小板被输注给25名患者，未出现并发症。因此，在输血前于22摄氏度下延长血小板的储存时间需要严格注意任何可能的外部污染来源，因为革兰氏阳性菌在这些条件下可能会增殖并导致受血者出现感染并发症。
