Sivas Cumhuriyet University, Faculty of Health Science, Department of Nutrition and Dietetics, Sivas 58140, Turkey.
Tokat M. Emin Sarac Anatolian Religious High School, Tokat 60030, Turkey.
Int J Med Mushrooms. 2023;25(6):75-86. doi: 10.1615/IntJMedMushrooms.2023047802.
Mushrooms, which have been collected to meet the nutritional needs of the world for many years, have gained medical importance thanks to the bioactive compounds they produce. Thanks to studies carried out to determine mushroom diversity, the number of species identified is increasing year by year. Accordingly, in recent years, studies conducted to determine the biological activities of metabolites produced by fungi have been increasing. The present study was conducted to determine the cytotoxic, antioxidant, antibiofilm and antimicrobial activities of the seven different mushroom species (Craterellus cornucopioides, Hymenopellis radicata, Lepista nuda, Pisolithus arhizus, Ramaria flava, Schizophyllum commune, and Tricholoma ustale) collected from Tokat and Yozgat regions located in northern and central Turkey. Laboratory studies have demonstrated that mushrooms used in this study have different degrees of antibiofilm, antimicrobial, antioxidant and cytotoxic activities. At the end of the study, it is determined that C. cornucopioides and L. nuda species have the highest antimicrobial activity. In addition, mushroom species have biofilm inhibitory effects on indicator microorganisms at varying degrees ranging between 20.7 and 96.3%. As a result of antioxidant activity studies, it was determined that T. ustale has the highest free radical scavenging effect and P. arhizus, which has the highest polyphenol content, has the highest reducing power. Finally, it is determined that, among the mushrooms used in the present study, H. radicata showed higher selectivity on the MDA-MB-231 breast cancer cell line than on the normal cell line tested, while C. cornucopioides showed higher selectivity on the MCF-7 breast cancer cell line.
多年来，人们一直收集蘑菇以满足世界的营养需求，由于其产生的生物活性化合物，蘑菇的医学重要性日益增加。由于开展了确定蘑菇多样性的研究，每年鉴定的物种数量都在增加。因此，近年来，为确定真菌产生的代谢物的生物活性而进行的研究越来越多。本研究旨在确定从土耳其北部和中部的托卡特和约兹加特地区收集的七种不同蘑菇（Cornucopioides 蕈、Hymenopellis radicata、Lepista nuda、Pisolithus arhizus、Ramaria flava、Schizophyllum commune 和 Tricholoma ustale）的细胞毒性、抗氧化、抗生物膜和抗菌活性。实验室研究表明，本研究中使用的蘑菇具有不同程度的抗生物膜、抗菌、抗氧化和细胞毒性活性。研究结束时，确定 C. cornucopioides 和 L. nuda 物种具有最高的抗菌活性。此外，蘑菇物种对指示微生物的生物膜抑制作用在 20.7%至 96.3%之间的不同程度上具有不同程度的抑制作用。抗氧化活性研究结果表明，T. ustale 具有最高的自由基清除效果，而多酚含量最高的 P. arhizus 具有最高的还原能力。最后，确定在所研究的蘑菇中，H. radicata 对 MDA-MB-231 乳腺癌细胞系的选择性高于测试的正常细胞系，而 C. cornucopioides 对 MCF-7 乳腺癌细胞系的选择性更高。
