Tang Chuyu, Fan Yuejun, Wang Tao, Wang Jie, Xiao Mengjun, He Min, Chang Xiyun, Li Yuling, Li Xiuzhang
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining 810016, China.
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, China.
Antioxidants (Basel). 2024 May 20;13(5):620. doi: 10.3390/antiox13050620.
, an endemic resource of the Tibetan Plateau, possesses significant medicinal and ecological values. However, the understanding of antioxidant capacity and metabolic profiling of from diverse regions remains elusive due to limited resources. Therefore, to comprehensively comprehend the antioxidant capacity and metabolite diversity of , we conducted a rounded analysis of its antioxidant capacity from three distinct regions using both untargeted and targeted metabolomics. Determination of antioxidant indices, such as ferric ion-reducing antioxidant power (FRAP), total phenolic content (TPC), and flavonoid content (FC), revealed the robust antioxidant capacity of . QL (QLFL) exhibited no significant difference compared to ZD (ZDFL); however, both were significantly distinct from XH (XHFL) across multiple indices. Furthermore, a positive correlation was observed between FRAP and flavonoid content. A total of 5782 metabolites were identified and chemically classified. Metabolites of varied significantly at different regions and eight key differential metabolites were screened. Phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, and cyanoamino acid metabolism were the main different regulatory pathways. Consequently, the disparities in the antioxidant activity of may primarily be ascribed to the biosynthesis and metabolism of phenylalanine, while vanillic acid could potentially serve as a pivotal metabolite influencing the antioxidative capacity of by targeted metabolomics. These findings enhance our understanding of the composition of and provide valuable resources for its comprehensive utilization and targeted development.
作为青藏高原的一种特有资源，具有重要的药用和生态价值。然而，由于资源有限，对于来自不同地区的[具体名称未给出]的抗氧化能力和代谢谱的了解仍然不足。因此，为了全面了解[具体名称未给出]的抗氧化能力和代谢物多样性，我们使用非靶向和靶向代谢组学方法，对来自三个不同地区的[具体名称未给出]的抗氧化能力进行了全面分析。通过测定抗氧化指标，如铁离子还原抗氧化能力（FRAP）、总酚含量（TPC）和黄酮类化合物含量（FC），揭示了[具体名称未给出]强大的抗氧化能力。QL[具体名称未给出]（QLFL）与ZD[具体名称未给出]（ZDFL）相比无显著差异；然而，在多个指标上，两者均与XH[具体名称未给出]（XHFL）有显著差异。此外，还观察到FRAP与黄酮类化合物含量之间存在正相关。共鉴定出5782种代谢物并进行了化学分类。[具体名称未给出]的代谢物在不同地区差异显著，并筛选出了8种关键差异代谢物。苯丙氨酸、酪氨酸和色氨酸的生物合成、苯丙氨酸代谢以及氰基氨基酸代谢是主要的不同调控途径。因此，[具体名称未给出]抗氧化活性的差异可能主要归因于苯丙氨酸的生物合成和代谢，而香草酸可能是通过靶向代谢组学影响[具体名称未给出]抗氧化能力的关键代谢物。这些发现增进了我们对[具体名称未给出]成分的理解，并为其综合利用和靶向开发提供了有价值的资源。
