Marker J C, Arnall D A, Conlee R K, Winder W W
Am J Physiol. 1986 Sep;251(3 Pt 2):R552-9. doi: 10.1152/ajpregu.1986.251.3.R552.
To determine the role of epinephrine in glycogenolysis during high-intensity exercise, rats were adrenodemedullated (ADM) or sham operated (SHAM) and run for either 30 min at 38 m/min or for 5 min at 27, 38, or 48 m/min up a 15% grade. At the end of exercise the rats were anesthetized by intravenous injection of pentobarbital sodium. Liver, blood, and muscle samples were obtained. Plasma epinephrine values were 5.9 and 0.3 nM for SHAM and ADM animals, respectively, after 30 min of exercise. Liver glycogen decreased by 16 and 21 mg/g in the SHAM and ADM groups, respectively, and liver cAMP increased significantly in both groups. Glycogen in the soleus muscle decreased 80% in the SHAM but only 43% in the ADM animals after 30 min of exercise. The exercise-induced hyperglycemia observed in the SHAM animals was not present in the ADM animals. The responses of cyclic AMP, soleus glycogen, and blood glucose were similar in both the 5- and 30-min exercise groups. During intense exercise, epinephrine is unessential for stimulating liver glycogenolysis but does play an important role in stimulating glycogenolysis in the soleus muscle and in establishing exercise-induced hyperglycemia.
为了确定肾上腺素在高强度运动期间糖原分解中的作用，将大鼠进行肾上腺髓质切除（ADM）或假手术（SHAM），然后以38米/分钟的速度跑30分钟，或以27、38或48米/分钟的速度在15%坡度上跑5分钟。运动结束时，通过静脉注射戊巴比妥钠对大鼠进行麻醉。获取肝脏、血液和肌肉样本。运动30分钟后，SHAM组和ADM组动物的血浆肾上腺素值分别为5.9和0.3纳摩尔/升。SHAM组和ADM组肝脏糖原分别减少了16和21毫克/克，两组肝脏环磷酸腺苷（cAMP）均显著增加。运动30分钟后，SHAM组比目鱼肌糖原减少80%，而ADM组动物仅减少43%。SHAM组动物出现的运动诱导的高血糖在ADM组动物中未出现。5分钟和30分钟运动组中，环磷酸腺苷、比目鱼肌糖原和血糖的反应相似。在剧烈运动期间，肾上腺素对于刺激肝脏糖原分解并非必不可少，但在刺激比目鱼肌糖原分解和引发运动诱导的高血糖方面确实发挥重要作用。
