Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong 266237, China.
Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong 266237, China.
Bioelectrochemistry. 2023 Dec;154:108526. doi: 10.1016/j.bioelechem.2023.108526. Epub 2023 Jul 21.
In this study, a 13-mer antimicrobial peptide (RRWRIVVIRVRRC) named by E6 was used as an enhancer of a green biocide to mitigate the biocorrosion of EH36 ship steel. Results show that a low concentration of E6 (100 nM) alone was no-biocidal and could not resist the Desulfovibrio vulgaris adhesion on the EH36 steel surface. However, E6 enhanced the bactericidal effect of tetrakis hydroxymethyl phosphonium sulfate (THPS). When E6 and THPS were both added to the bacteria and steel system, both the sessile D. vulgaris cells and biocorrosion rate of EH36 steel decreased significantly. Compared with the 80 ppm THPS alone treatment, the combination of 100 nM E6 + 80 ppm THPS led to an extra 1.6-log reduction in the sessile cell count. Fewer sessile D. vulgaris cells led to a lower extracellular electron transfer (EET) rate, directly resulting in 78% and 83% decreases in weight loss and pit depth of EH36 steel, respectively. E6 saved more than 50% of THPS dosage in this work to achieve a similar biocorrosion mitigation effect on EH36 steel.
在这项研究中，一种名为 E6 的 13 肽抗菌肽（RRWRIVVIRVRRC）被用作绿色杀生剂的增效剂，以减轻 EH36 船用钢的生物腐蚀。结果表明，低浓度的 E6（100 nM）本身没有杀菌作用，不能抵抗普通脱硫弧菌在 EH36 钢表面的附着。然而，E6 增强了四羟甲基硫酸磷（THPS）的杀菌作用。当 E6 和 THPS 同时添加到细菌和钢体系中时，附着的脱硫弧菌细胞和 EH36 钢的生物腐蚀速率都显著降低。与单独使用 80 ppm THPS 相比，组合使用 100 nM E6 和 80 ppm THPS 使附着细胞数量额外减少了 1.6 个对数。附着的脱硫弧菌细胞越少，细胞外电子转移（EET）速率越低，直接导致 EH36 钢的失重和蚀坑深度分别降低了 78%和 83%。E6 在这项工作中节省了超过 50%的 THPS 用量，从而对 EH36 钢达到了类似的生物腐蚀缓解效果。
