Wang Di, Unsal Tuba, Kumseranee Sith, Punpruk Suchada, Saleh Mazen A, Alotaibi Mohammed D, Xu Dake, Gu Tingyue
Shenyang National Lab for Materials Science, Northeastern University, Shenyang, 110819, China.
Department of Chemical & Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, 45701, USA.
Bioresour Bioprocess. 2022 Jun 13;9(1):67. doi: 10.1186/s40643-022-00553-z.
Biocorrosion, also called microbiologically influenced corrosion (MIC), is a common operational threat to many industrial processes. It threatens carbon steel, stainless steel and many other metals. In the bioprocessing industry, reactor vessels in biomass processing and bioleaching are prone to MIC. MIC is caused by biofilms. The formation and morphology of biofilms can be impacted by fluid flow. Fluid velocity affects biocide distribution and MIC. Thus, assessing the efficacy of a biocide for the mitigation of MIC under flow condition is desired before a field trial. In this work, a benchtop closed flow loop bioreactor design was used to investigate the biocide mitigation of MIC of C1018 carbon steel at 25 °C for 7 days using enriched artificial seawater. An oilfield biofilm consortium was analyzed using metagenomics. The biofilm consortium was grown anaerobically in the flow loop which had a holding vessel for the culture medium and a chamber to hold C1018 carbon steel coupons. Peptide A (codename) was a chemically synthesized cyclic 14-mer (cys-ser-val-pro-tyr-asp-tyr-asn-trp-tyr-ser-asn-trp-cys) with its core 12-mer sequence originated from a biofilm dispersing protein secreted by a sea anemone which possesses a biofilm-free exterior. It was used as a biocide enhancer. The combination of 50 ppm (w/w) THPS (tetrakis hydroxymethyl phosphonium sulfate) biocide + 100 nM (180 ppb by mass) Peptide A resulted in extra 1-log reduction in the sulfate reducing bacteria (SRB) sessile cell count and the acid producing bacteria (APB) sessile cell count compared to 50 ppm THPS alone treatment. Furthermore, with the enhancement of 100 nM Peptide A, extra 44% reduction in weight loss and 36% abatement in corrosion pit depth were achieved compared to 50 ppm THPS alone treatment.
生物腐蚀，也称为微生物影响腐蚀（MIC），是许多工业过程中常见的运行威胁。它会威胁碳钢、不锈钢和许多其他金属。在生物加工行业中，生物质加工和生物浸出中的反应容器容易受到微生物影响腐蚀。微生物影响腐蚀是由生物膜引起的。生物膜的形成和形态会受到流体流动的影响。流体速度会影响杀生剂的分布和微生物影响腐蚀。因此，在进行现场试验之前，需要评估杀生剂在流动条件下减轻微生物影响腐蚀的效果。在这项工作中，采用了台式封闭流动回路生物反应器设计，以研究在25℃下使用富集人工海水，杀生剂对C1018碳钢微生物影响腐蚀的减轻作用，为期7天。使用宏基因组学分析了一个油田生物膜群落。该生物膜群落在流动回路中厌氧生长，该流动回路有一个用于培养基的储存容器和一个用于放置C1018碳钢试片的腔室。肽A（代号）是一种化学合成的环状十四肽（半胱氨酸-丝氨酸-缬氨酸-脯氨酸-酪氨酸-天冬氨酸-酪氨酸-天冬酰胺-色氨酸-酪氨酸-丝氨酸-天冬酰胺-色氨酸-半胱氨酸），其核心十二肽序列源自海葵分泌的一种生物膜分散蛋白，该海葵表面无生物膜。它被用作杀生剂增强剂。与单独使用50 ppm（w/w）的四羟甲基硫酸磷（THPS）杀生剂处理相比，50 ppm（w/w）的THPS杀生剂+100 nM（质量分数为180 ppb）的肽A组合使硫酸盐还原菌（SRB）的固着细胞数量和产酸菌（APB）的固着细胞数量额外减少了1个对数级。此外，与单独使用50 ppm THPS处理相比，在添加100 nM肽A后，失重额外减少了44%，腐蚀坑深度降低了36%。
