Department of Chemical and Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, OH, 45701, USA.
Ziolase, LLC, Winter Springs, FL, 32708, USA.
Bioprocess Biosyst Eng. 2022 Apr;45(4):659-667. doi: 10.1007/s00449-021-02684-7. Epub 2022 Jan 4.
Trehalase can biocatalyze the conversion of trehalose to glucose. It is an enzyme that plays an important role in biofilm formation. Thus, trehalase has been patented as a chemical for preventing and treating biofilms. Sulfate-reducing bacteria (SRB) biofilms are often found responsible for biocorrosion, also known as microbiologically infuenced corrosion (MIC), especially in the oil and gas industries and in water utilities. The MIC treatment process typically requires biocide treatment of biofilms, sometimes together with scrubbing. Owing to environmental concerns, a lower biocide dosage is desired in the treatment process. In this work, trehalase was tested as a green biocide enhancer to enhance tetrakis hydroxymethyl phosphonium sulfate (THPS) in the prevention of Desulfovibrio vulgaris MIC of C1018 carbon steel in ATCC 1249 culture medium at 37 °C. THPS is one of the most popular industrial biocides owing to its broad-spectrum efficacy and green chemical status. After 7 days of incubation in 50 mL anaerobic vials containing 40 mL culture medium at pH 7.0, the sessile cell counts indicated that 50 ppm (w/w) THPS + 30 ppm (w/w) trehalase led to an extra 5.7-fold sessile cell reduction when compared with the 50 ppm THPS alone treatment. As a consequence, the combination treatment also resulted in an extra 54% in pit depth reduction and 30% in weight loss reduction when compared with the 50 ppm THPS alone treatment (with 9.0 μm and 1.0 mg/cm). The biofilm images corroborated the decreased sessile cell count and pitting corrosion.
海藻糖酶可以催化海藻糖转化为葡萄糖。它是一种在生物膜形成中起重要作用的酶。因此，海藻糖酶已被专利为一种用于预防和治疗生物膜的化学物质。硫酸盐还原菌 (SRB) 生物膜通常被认为是造成生物腐蚀的原因，也称为微生物影响腐蚀 (MIC)，特别是在石油和天然气行业以及水公用事业中。MIC 处理过程通常需要对生物膜进行杀菌剂处理，有时还需要进行擦洗。由于环境方面的考虑，希望在处理过程中减少杀菌剂的用量。在这项工作中，海藻糖酶被测试为一种绿色杀菌剂增强剂，以增强四羟甲基氯化鏻 (THPS) 在预防 Desulfovibrio vulgaris 中对 C1018 碳钢的 MIC 的作用，在 ATCC 1249 培养基中 37°C。THPS 是最受欢迎的工业杀菌剂之一，因为它具有广谱功效和绿色化学状态。在含有 40mL 培养基的 50mL 厌氧小瓶中孵育 7 天后，静止细胞计数表明，与单独使用 50ppm THPS 相比，50ppm (w/w) THPS+30ppm (w/w) 海藻糖酶导致静止细胞减少了 5.7 倍。因此，与单独使用 50ppm THPS 相比，组合处理还导致凹坑深度减少了 54%，重量损失减少了 30%（分别为 9.0μm 和 1.0mg/cm）。生物膜图像证实了静止细胞计数和点蚀腐蚀的减少。
