Xu Lingjun, Kijkla Pruch, Kumseranee Sith, Punpruk Suchada, Gu Tingyue
Department of Chemical & Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, OH 45701, USA.
PTT Exploration and Production, Bangkok 10900, Thailand.
Antibiotics (Basel). 2023 Jul 15;12(7):1194. doi: 10.3390/antibiotics12071194.
MIC (microbiologically influenced corrosion) is problematic in many industries, especially in the oil and gas industry. In this work, N80 carbon steel for pipelines was tested with 26Cr3Mo chromium pipeline steel for comparison in SRB (sulfate-reducing bacterium) MIC mitigation using a THPS (tetrakis hydroxymethyl phosphonium sulfate)-based commercial biocide (Biotreat 5475 with 75-80% THPS by mass). Peptide A, a nature-mimicking synthetic cyclic peptide (cys-ser-val-pro-tyr-asp-tyr-asn-trp-tyr-ser-asn-trp-cys) with biofilm dispersal ability was used as a biocide enhancer. Metal coupons covered with 3-d old IS5 biofilms were immersed in different biocide solutions. After 1-h treatment, 200 ppm Biotreat 5475, 200 ppm Biotreat 5475 + 200 nM (360 ppb) Peptide A, and 400 ppm Biotreat 5475 achieved 0.5-log, 1.7-log and 1.9-log reductions in sessile cell count on N80, and 0.7-log, 1.7-log, and 1.8-log on 26Cr3Mo, respectively. The addition of 200 nM Peptide A cut the THPS biocide dosage by nearly half. Biocide injection tests in electrochemical glass cells after 1 h exhibited 15%, 70%, and 72% corrosion inhibition efficiency (based on corrosion current density) on N80, and 27%, 79%, 75% on 26Cr3Mo, respectively. Linear polarization resistance and electrochemical impedance spectrometry results also indicated antimicrobial efficacies.
微生物影响的腐蚀（MIC）在许多行业中都是一个问题，尤其是在石油和天然气行业。在这项工作中，使用基于四羟甲基硫酸鏻（THPS）的商用杀菌剂（质量分数为75 - 80%的Biotreat 5475），对管道用N80碳钢与26Cr3Mo铬管道钢进行了硫酸盐还原菌（SRB）导致的MIC缓解测试比较。肽A是一种具有生物膜分散能力的模拟天然合成环肽（半胱氨酸 - 丝氨酸 - 缬氨酸 - 脯氨酸 - 酪氨酸 - 天冬氨酸 - 酪氨酸 - 天冬酰胺 - 色氨酸 - 酪氨酸 - 丝氨酸 - 天冬酰胺 - 色氨酸 - 半胱氨酸），用作杀菌剂增强剂。将覆盖有3天龄IS5生物膜的金属试片浸入不同的杀菌剂溶液中。经过1小时处理后，200 ppm的Biotreat 5475、200 ppm的Biotreat 5475 + 200 nM（360 ppb）肽A和400 ppm的Biotreat 5475分别使N80上的固着细胞数量减少了0.5个对数级、1.7个对数级和1.9个对数级，使26Cr3Mo上的固着细胞数量分别减少了0.7个对数级、1.7个对数级和1.8个对数级。添加200 nM肽A可使THPS杀菌剂用量减少近一半。1小时后在电化学玻璃池中进行的杀菌剂注入测试表明，基于腐蚀电流密度，对N80的缓蚀效率分别为15%、70%和72%，对26Cr3Mo的缓蚀效率分别为27%、79%和75%。线性极化电阻和电化学阻抗谱结果也表明了抗菌效果。
