基于 pH 响应型聚合物深共晶溶剂的双水相体系用于高效萃取芳香族氨基酸。
Aqueous two-phase system based on pH-responsive polymeric deep eutectic solvent for efficient extraction of aromatic amino acids.
机构信息
Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
出版信息
Food Chem. 2024 Jan 1;430:137029. doi: 10.1016/j.foodchem.2023.137029. Epub 2023 Jul 27.
Recently, more and more attention has been paid to the construction of stimulus-responsive aqueous two-phase systems (ATPSs) for the extraction and separation of various bioactive compounds. In this work, an ATPS based on a pH-responsive polymeric deep eutectic solvent (PDES) and phosphate salt was constructed for the first time. The pH-response properties of the PDES were studied through a series of experiments. Additionally, the phase formation mechanism was studied through experiments and simulations. This novel PDES-based ATPS was used to extract aromatic amino acids (AAAs). The extraction efficiencies for tyrosine (Tyr), phenylalanine (Phe), and tryptophan (Trp) reached 95.25%, 99.05%, and 99.10%, respectively. By adjusting pH, PDES was recycled and reused. This novel and recyclable PDES-based ATPS could be an efficient method for the extraction of AAAs, which could also be applied used as a versatile and sustainable method for the extraction of other bioactive compounds in the future.
最近，人们越来越关注刺激响应型双水相体系（ATPS）的构建，以用于各种生物活性化合物的提取和分离。在这项工作中，首次构建了基于 pH 响应型聚合型深共晶溶剂（PDES）和磷酸盐的 ATPS。通过一系列实验研究了 PDES 的 pH 响应特性。此外，通过实验和模拟研究了相形成机制。该新型基于 PDES 的 ATPS 用于提取芳香族氨基酸（AAAs）。酪氨酸（Tyr）、苯丙氨酸（Phe）和色氨酸（Trp）的萃取效率分别达到 95.25%、99.05%和 99.10%。通过调节 pH 值，PDES 可以回收和再利用。这种新型的可回收 PDES 基 ATPS 可能是提取 AAAs 的有效方法，未来也可能作为提取其他生物活性化合物的通用和可持续方法。
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