Hunan Electronic Information Industry Institute, Changsha 410012, China.
Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Molecules. 2022 Aug 19;27(16):5288. doi: 10.3390/molecules27165288.
(1) Background: Aqueous two-phase systems (ATPSs) have been widely used in the separation and purification of bioactive substances in recent years. (2) Methods: In this study, deep eutectic solvents (DESs)-based ATPSs were employed for the extraction and separation of polysaccharides (AMP). The optimal DES (choline chloride:urea = 1:1) was first screened to extract AMP, and the effect of DES concentration, solid-liquid ratio, pH, extraction temperature, and extraction time on the extraction yield of AMP were investigated. (3) Results: The maximum extraction yield was 141.11 mg/g under the optimum conditions. AMP was then preliminarily purified by ATPS, to further realize the recycling and reuse of DES. The effect of type of salts, salt concentration, and extraction temperature on extraction efficiency was investigated. The extraction efficiency was 97.85% for AMP under the optimum ATPS conditions. Finally, the obtained AMP was studied by molecular weight determination, infrared spectroscopy analysis, and monosaccharide composition analysis. (4) Conclusions: This ATPS extraction based on DESs is simple, environmentally friendly, low-cost, and has high extraction efficiency, which provides new ideas for the extraction of plant polysaccharides and other bioactive compounds.
(1) 背景：近年来，双水相体系（ATPS）已广泛应用于生物活性物质的分离和纯化。
(2) 方法：本研究采用基于深共熔溶剂（DES）的 ATPS 提取和分离多糖（AMP）。首先筛选出最优的 DES（氯化胆碱：尿素=1:1）来提取 AMP，并考察了 DES 浓度、固液比、pH 值、提取温度和提取时间对 AMP 提取率的影响。
(3) 结果：在最佳条件下，AMP 的最大提取率为 141.11mg/g。然后通过 ATPS 对 AMP 进行初步纯化，以进一步实现 DES 的回收和再利用。考察了盐的类型、盐浓度和提取温度对提取效率的影响。在最佳 ATPS 条件下，AMP 的提取效率为 97.85%。最后，通过分子量测定、红外光谱分析和单糖组成分析对提取得到的 AMP 进行了研究。
(4) 结论：这种基于 DES 的 ATPS 提取方法简单、环保、低成本、提取效率高，为植物多糖等生物活性物质的提取提供了新的思路。
