School of Life Science, Xinghuacun College (Shanxi Institute of Brewing Technology and Industry), Shanxi University, Taiyuan 030006, China.
Institute of Pharmaceutical and Food Engineering, Shanxi University of Chinese Medicine, Yuci 030619, China.
Food Chem. 2024 Jan 1;430:136904. doi: 10.1016/j.foodchem.2023.136904. Epub 2023 Jul 20.
An ingenious and universal design of fluorescent/electrochemical dual-signal mode sensing platform was constructed for the sensitive, selective and accurate detection of tetracycline (TET). Apt-functionalized nano-magnetic beads (FeO-Apt) as capture probe, Apt-complementary short-chain functionalized fluorescent MOF loaded with methylene blue (MB) (cDNA-MOF-MB) as dual-signal tag were prepared. The sensing platform (FeO-Apt/cDNA-MOF-MB) was formed based on the base complementary pairing of Apt and cDNA. With the help of Apt for target recognition, together with magnetic separation technology, a dual-signal mode biosensor was constructed. The dual-signal mode biosensor exhibited a wide linear concentration range from 1.00 × 10 g/mL to 1.00 × 10 g/mL with a low limit of detection (LOD) of 1.69 × 10 g/mL (fluorescence mode assay) and 1.15 × 10 g/mL (electrochemical mode assay). The proposed biosensor had been successfully applied to the determination of TET content in real samples with satisfactory recoveries (94.99-101.30%).
构建了一种用于四环素（TET）灵敏、选择性和准确检测的荧光/电化学双信号模式传感平台的巧妙且通用的设计。将功能化的纳米磁珠（FeO-Apt）作为捕获探针，将与甲基蓝（MB）负载的 Apt 互补短链功能化荧光 MOF（cDNA-MOF-MB）作为双信号标记物进行制备。基于 Apt 和 cDNA 的碱基互补配对，形成传感平台（FeO-Apt/cDNA-MOF-MB）。借助 Apt 进行目标识别，并结合磁分离技术，构建了双信号模式生物传感器。双信号模式生物传感器在 1.00×10 g/mL 至 1.00×10 g/mL 的宽线性浓度范围内表现出良好的线性关系，检测限（LOD）低至 1.69×10 g/mL（荧光模式检测）和 1.15×10 g/mL（电化学模式检测）。该生物传感器已成功应用于实际样品中 TET 含量的测定，回收率令人满意（94.99-101.30%）。
