Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, USA.
Department of Physical Medicine and Rehabilitation, University of Utah, Salt Lake City, UT, USA.
J Neurosci Methods. 2023 Aug 1;396:109934. doi: 10.1016/j.jneumeth.2023.109934. Epub 2023 Jul 29.
The study of chronic pain and its treatments requires a robust animal model with objective and quantifiable metrics. Porcine neuropathic pain models have been assessed with peripheral pain recordings and behavioral responses, but thus far central nervous system electrophysiology has not been investigated. This work aimed to record non-invasive, somatosensory-evoked potentials (SEPs) via electroencephalography in order to quantitatively assess chronic neuropathic pain induced in a porcine model.
Peripheral neuritis trauma (PNT) was induced unilaterally in the common peroneal nerve of domestic farm pigs, with the contralateral leg serving as the control for each animal. SEPs were generated by stimulation of the peripheral nerves distal to the PNT and were recorded non-invasively using transcranial electroencephalography (EEG). The P30 wave of the SEP was analyzed for latency changes.
P30 SEPs were successfully recorded with non-invasive EEG. PNT resulted in significantly longer P30 SEP latencies (p < 0.01 [n = 8]) with a median latency increase of 14.3 [IQR 5.0 - 17.5] ms. Histological results confirmed perineural inflammatory response and nerve damage around the PNT nerves.
COMPARISON WITH EXISTING METHOD(S): Control P30 SEPs were similar in latency and amplitude to those previously recorded invasively in healthy pigs. Non-invasive recordings have numerous advantages over invasive measures.
P30 SEP latency can serve as a quantifiable neurological measure that reflects central nervous system processing in a porcine model of chronic pain. Advancing the development of a porcine chronic pain model will facilitate the translation of experimental therapies into human clinical trials.
慢性疼痛及其治疗的研究需要一个具有客观和可量化指标的强大动物模型。猪神经性疼痛模型已经通过外周疼痛记录和行为反应进行了评估，但迄今为止尚未研究中枢神经系统电生理学。这项工作旨在通过脑电图记录非侵入性、体感诱发电位（SEP），以便定量评估在猪模型中诱导的慢性神经性疼痛。
在国内农场猪的腓总神经单侧诱导周围神经炎创伤（PNT），每只动物的对侧腿作为对照。SEP 由 PNT 远端外周神经刺激产生，并使用经颅脑电图（EEG）进行非侵入性记录。分析 SEP 的 P30 波潜伏期变化。
使用非侵入性 EEG 成功记录了 P30 SEP。PNT 导致 P30 SEP 潜伏期显著延长（p<0.01 [n=8]），中位潜伏期增加 14.3 [IQR 5.0-17.5] ms。组织学结果证实了 PNT 神经周围的炎症反应和神经损伤。
潜伏期和振幅相似的对照 P30 SEP 与以前在健康猪中侵入性记录的 SEP 相似。非侵入性记录具有许多优于侵入性测量的优势。
P30 SEP 潜伏期可以作为一种可量化的神经学测量指标，反映猪慢性疼痛模型中中枢神经系统的处理情况。推进猪慢性疼痛模型的发展将有助于将实验疗法转化为人类临床试验。
