Rothammer Maximilian, Strobel Philipp, Zollfrank Cordt, Urmann Corinna
Chair for Biogenic Polymers, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany.
TUM Campus Straubing, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany; Organic-Analytical Chemistry, Weihenstephen-Triesdorf University of Applied Sciences, Schulgasse 16, 94315 Straubing, Germany.
Int J Biol Macromol. 2023 Oct 1;250:126063. doi: 10.1016/j.ijbiomac.2023.126063. Epub 2023 Jul 29.
Materials derived from renewable resources have great potential to replace fossil-based plastics in biomedical applications. In this study, the synthesis of cellulose-based photoresists by esterification with methacrylic acid anhydride and sorbic acid was investigated. These resists polymerize under UV irradiation in the range of λ = 254 nm to 365 nm, with or, in the case of the sorbic acid derivative, without using an additional photoinitiator. Usability for biomedical applications was demonstrated by investigating the adhesion and viability of a fibrosarcoma cell line (HT-1080). Compared to polystyrene, the material widely used for cell culture dishes, cell adhesion to the biomaterials tested was even stronger, as assessed by a centrifugation assay. Remarkably, chemical surface modifications of cellulose acetate with methacrylate and sorbic acid allow direct attachment of HT-1080 cells without adding protein modifiers or ligands. Furthermore, cells on both biomaterials show similar cell viability, not significantly different from polystyrene, indicating no significant impairment or enhancement, allowing the use of these cellulose derivatives as support structures for scaffolds or as a self-supporting coating for cell culture solely based on renewable resources.
源自可再生资源的材料在生物医学应用中具有巨大潜力，可替代基于化石的塑料。在本研究中，对通过与甲基丙烯酸酐和山梨酸进行酯化反应合成纤维素基光刻胶进行了研究。这些光刻胶在λ = 254 nm至365 nm范围内的紫外线照射下聚合，对于山梨酸衍生物，使用或不使用额外的光引发剂均可。通过研究纤维肉瘤细胞系（HT - 1080）的粘附性和活力，证明了其在生物医学应用中的适用性。与广泛用于细胞培养皿的聚苯乙烯相比，通过离心试验评估，测试的生物材料对细胞的粘附力更强。值得注意的是，用甲基丙烯酸酯和山梨酸对醋酸纤维素进行化学表面改性，无需添加蛋白质改性剂或配体即可直接附着HT - 1080细胞。此外，两种生物材料上的细胞显示出相似的细胞活力，与聚苯乙烯无显著差异，表明没有明显的损害或增强，这使得这些纤维素衍生物可以用作支架的支撑结构或仅基于可再生资源的细胞培养自支撑涂层。
