Institute for Particle Technology, Technische Universität Braunschweig, Volkmaroder Straße 5, D-38104 Braunschweig, Germany.
Lett Appl Microbiol. 2023 Aug 2;76(8). doi: 10.1093/lambio/ovad089.
Industrial biotechnology uses microbial cells to produce a wide range of products. While the genetic and molecular properties of these organisms are well understood, less is known about their mechanical properties. Previous work has established a test procedure for single yeast cells using a nanoindentation instrument equipped with a flat-punch probe, which allows single cells (Saccharomyces cerevisiae) to be compressed between two parallel surfaces. The resulting force-displacement curves clearly showed the bursting of the cells and were used to determine characteristics such as burst force and burst energy. Other studies have investigated the influence of growth conditions and measurement conditions on the mechanical characteristics. The recent study examined the mechanical characteristics according to the temperature during compression. Temperature from 0°C to 25°C has no significant effect on the micromechanical properties. Increasing the temperature up to 35°C causes a reduction in the strength of the cells. At even higher temperatures, up to 50°C, the burst force and burst energy increase significantly. A deformation geometry model was used to calculate the cell wall tensile strength as a function of temperature. The results of these studies may facilitate the identification of efficient conditions for cell disruption and product recovery in downstream biotechnological processes.
工业生物技术利用微生物细胞生产各种产品。尽管这些生物体的遗传和分子特性已经得到很好的理解，但它们的机械特性却知之甚少。先前的工作已经建立了一种使用配备平头探针的纳米压痕仪器对单个酵母细胞进行测试的程序，该程序允许将单个细胞（酿酒酵母）压缩在两个平行表面之间。由此产生的力-位移曲线清楚地显示了细胞的爆裂，并用于确定爆裂力和爆裂能等特性。其他研究还研究了生长条件和测量条件对机械特性的影响。最近的研究根据压缩过程中的温度研究了机械特性。温度从 0°C 到 25°C 对微机械性能没有显著影响。将温度升高到 35°C 会导致细胞强度降低。在更高的温度下，高达 50°C，爆裂力和爆裂能显著增加。使用变形几何模型计算了细胞壁拉伸强度随温度的变化。这些研究的结果可能有助于确定下游生物技术过程中细胞破碎和产物回收的有效条件。
