Microbiology Department, Applied Biotechnology Section, Faculty of Science, Ain Shams University, Cairo, Egypt.
Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.
Microb Cell Fact. 2023 Aug 1;22(1):142. doi: 10.1186/s12934-023-02152-9.
Malachite Green (MG) dye of the triphenylmethane group is a toxic compound used in the aquaculture industry as an antifungal agent, however, it can accumulate in fish and pose toxicity. The present work aims to remove MG in Microbial Fuel Cell (MFC) as a sustainable and eco-friendly solution. Out of six samples, the highest malachite green degradation was obtained by a sample obtained from Robiki tannery site in agar plates in 24 h at 37 °C. Robiki sample was used to inoculate the anodic chamber in Microbial Fuel cell, the resulting average electricity production was 195.76 mV for two weeks. The decolorization average was almost 88%. The predominant bacteria responsible for MG decolorization and electricity production were identified using 16S rRNA as Shewanella chilikensis strain MG22 (Accession no. OP795826) and formed a heavy biofilm on the anode. At the end of the decolorization process, MG was added again for re-use of water. The results showed efficiency for re-use 3 times. To ensure the sterility of treated water for re-use, both UV and filter sterilization were used, the latter proved more efficient. The obtained results are promising, MFC can be used as recirculating aquaculture system (RAS). The same aquaculture water can be treated multiple times which provides a sustainable solution for water conservation.
孔雀石绿（MG）是三苯甲烷类染料的一种，作为一种抗真菌剂被应用于水产养殖行业，但其可在鱼类体内蓄积并具有毒性。本研究旨在利用微生物燃料电池（MFC）去除 MG，这是一种可持续且环保的解决方案。在 6 个样本中，在琼脂平板中于 37°C 下 24 小时获得了来自 Robiki 制革厂样品的最高孔雀石绿降解率。Robiki 样品被用于接种微生物燃料电池的阳极室，在两周内产生的平均电量为 195.76 mV。脱色平均率接近 88%。使用 16S rRNA 鉴定出负责 MG 脱色和发电的主要细菌为 Shewanella chilikensis 菌株 MG22（注册号 OP795826），并在阳极上形成了厚厚的生物膜。在脱色过程结束时，再次添加 MG 以重复利用水。结果表明可重复使用 3 次。为确保再利用水的无菌性，同时使用了紫外线和过滤灭菌，后者证明更有效。所得结果很有前景，微生物燃料电池可用于循环水产养殖系统（RAS）。相同的养殖用水可多次处理，为节约用水提供了可持续的解决方案。
