基于代谢组和转录组分析的抗炎化合物的质量变异与生物合成。
Quality variation and biosynthesis of anti-inflammatory compounds for based on the metabolome and transcriptome analysis.
作者信息
Liu Xiaoying, Aimaier Alimu, Wang Weilan, Dong Yuliang, Han Peng, He Jiang, Mu Lihong, Wang Xinhui, Li Jinyao
机构信息
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China.
Key Laboratory of Uygur Medicine, Xinjiang Institute of Materia Medica, Urumqi, China.
出版信息
Front Plant Sci. 2023 Jul 17;14:1224073. doi: 10.3389/fpls.2023.1224073. eCollection 2023.
INTRODUCTION
L. fruits as edible and medicinal plant, has anti-inflammatory activities. The different morphological characteristics of fruits from Ili, Turpan, and Karamay may affect their anti-inflammatory components and functions.
METHODS
The anti-inflammatory activity of fruit was assessed using an LPS-induced inflammatory cell model. Furthermore, the differences in anti-inflammatory compounds were analyzed by metabolome and RNA-seq. Additionally, the anti-inflammatory mechanism was elucidated using network pharmacology.
RESULTS
In the study, we found that the 95% ethanol extracts (CSE) obtained from the three kinds of fruits showed remarkable anti-inflammatory effects both and . However, the CSE derived from Ili fruits significantly reduced CD86 levels on DCs. As a result of metabolomic analysis, the metabolic profiles of Ili fruits differed significantly from those of the other two habitats, which were consistent with transcriptome analysis. A total of 15 compounds exhibiting anti-inflammatory activity were subjected to screening, revealing a greater accumulation of flavonoids in the Turpan and Karamay districts. Notably, phenolic compounds were identified as the principal anti-inflammatory components in .
CONCLUSION
There were significant differences in the morphology, metabolites, transcriptional levels, and anti-inflammatory activity of from the three districts.
引言
L.果实作为可食用和药用植物，具有抗炎活性。来自伊犁、吐鲁番和克拉玛依的果实不同形态特征可能会影响其抗炎成分和功能。
方法
使用脂多糖诱导的炎症细胞模型评估果实的抗炎活性。此外，通过代谢组学和RNA测序分析抗炎化合物的差异。另外，利用网络药理学阐明抗炎机制。
结果
在本研究中，我们发现从三种果实中获得的95%乙醇提取物（CSE）在体内和体外均显示出显著的抗炎作用。然而，源自伊犁果实的CSE显著降低了树突状细胞上的CD86水平。代谢组学分析结果表明，伊犁果实的代谢谱与其他两个产地的果实有显著差异，这与转录组分析结果一致。共筛选出15种具有抗炎活性的化合物，结果显示吐鲁番和克拉玛依地区的黄酮类化合物积累量更高。值得注意的是，酚类化合物被确定为果实中的主要抗炎成分。
结论
来自三个地区的果实的形态、代谢产物、转录水平和抗炎活性存在显著差异。
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