Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Road, Shanghai 200040, China.
J Agric Food Chem. 2010 Dec 22;58(24):12717-21. doi: 10.1021/jf1034114. Epub 2010 Nov 24.
Caper (Capparis spinosa L.) fruits have been used as food as well as folk medicine in the treatment of inflammatory disorders, such as rheumatism. The present study was carried out to study the anti-inflammatory activities of C. spinosa L. fruit (CSF) aqueous extract and to isolate main phytochemicals from its bioactive fractions. The CSF aqueous extract were separated into three fractions (CSF1-CSF3) by macroporous adsorption resins. The fractions CSF2 and CSF3 effectively inhibited the carrageenan-induced paw edema in mice. Systematic fractionation and isolation from CSF2+3 led to the identification of 13 compounds (1-13). Their chemical structures were elucidated by spectroscopic analyses including nuclear magnetic resonance (NMR) and mass spectrometry (MS) and literature comparisons. Major compounds found in the bioactive fraction CSF2+3 are flavonoids, indoles, and phenolic acids. To our knowledge, 8 of these 13 compounds (1-4, 6-7, 10, and 13) were identified from caper fruits for the first time. The anti-inflammatory effects of these purified compounds are currently under investigation.
木鳖果（Capparis spinosa L.）的果实既可以作为食物，也可以作为民间药物，用于治疗炎症性疾病，如风湿。本研究旨在研究木鳖果（CSF）水提取物的抗炎活性，并从其生物活性部位中分离主要的植物化学物质。CSF 水提取物通过大孔吸附树脂分离成三个馏分（CSF1-CSF3）。馏分 CSF2 和 CSF3 可有效抑制角叉菜胶诱导的小鼠足肿胀。从 CSF2+3 进行系统的分段和分离，鉴定出 13 种化合物（1-13）。通过包括核磁共振（NMR）和质谱（MS）在内的光谱分析以及文献比较，阐明了它们的化学结构。在生物活性馏分 CSF2+3 中发现的主要化合物是类黄酮、吲哚和酚酸。据我们所知，这些化合物中有 8 种（1-4、6-7、10 和 13）是首次从木鳖果中鉴定出来的。这些纯化化合物的抗炎作用目前正在研究中。
