CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Cell Rep Methods. 2023 Jun 27;3(7):100513. doi: 10.1016/j.crmeth.2023.100513. eCollection 2023 Jul 24.
A characteristic clinical complication in cancer patients is the frequent incidence of thrombotic events. Numerous studies have shown hyperactive/activated platelets to be a critical earlier trigger for cancer-associated thrombus formation. However, there currently is no viable approach to monitor specific changes in tumor-associated platelet activity. Here, we describe a chromatograph-like microfluidic device that is highly sensitive to the activity status of peripheral circulating platelets in both tumor-bearing mice and clinical cancer patients. Our results show a strongly positive correlation between platelet activation status and tumor progression. Six-month follow-up data from advanced cancer patients reveal positive links between platelet activity level and thrombus occurrence rate, with a high predictive capacity of thrombotic events (AUC = 0.842). Our findings suggest that circulating platelet activity status determined by this microfluidic device exhibits sensitive, predictive potential for thrombotic events in cancer patients for directing well-timed antithrombosis treatment.
在癌症患者中，一种典型的临床并发症是血栓事件频繁发生。许多研究表明，活性/激活的血小板是癌症相关血栓形成的一个关键早期触发因素。然而，目前尚无可行的方法来监测与肿瘤相关的血小板活性的特定变化。在这里，我们描述了一种类似色谱的微流控装置，它对荷瘤小鼠和临床癌症患者外周循环血小板的活性状态非常敏感。我们的结果表明血小板激活状态与肿瘤进展之间存在强烈的正相关。来自晚期癌症患者的 6 个月随访数据显示，血小板活性水平与血栓发生率之间存在正相关，具有较高的血栓事件预测能力（AUC=0.842）。我们的研究结果表明，这种微流控装置确定的循环血小板活性状态对癌症患者的血栓事件具有敏感的预测潜力，可指导及时的抗血栓治疗。
