Safika Safika, Indrawati Agustin, Afif Usamah, Hidayat Rahmat, Sunartatie Titiek
Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia.
J Adv Vet Anim Res. 2023 Jun 30;10(2):228-236. doi: 10.5455/javar.2023.j673. eCollection 2023 Jun.
This study analyzes the mycobiome in wild and captive Sumatran orangutans.
Nine orangutan feces samples from the wild and nine from captivity were divided into three repeats from 11- to 15-year-olds in good health. The Illumina platform for analysis of ITS bioinformatics was used according to the Qiime2 and CCMetagen approaches.
Wild Sumatran orangutans include 53% Ascomycota, 38% uncultured fungi, and 4% Basidiomycota. Orangutans in captivity are 57% Ascomycota, 26% uncultured fungi, and 2% Basidiomycota. Based on genus level, uncultured (31%), (10%), (3%), (3%), (2%), (2%), and (2%) are found in wild orangutans. The most prevalent genus among captivity orangutans is (32%), followed by fungal sp. (11%), (18%), (2%), and (2%). According to the Chao1 diversity index and Shannon and Simpson, there was no significant difference between wild and captive Sumatran orangutans.
is unique to wild Sumatran orangutans, although predominates in captive orangutans. We hypothesize that the gut mycobiome of wild orangutans will resemble that of orangutans in captivity. The excellent range of food sources in the forest does not result in the prevalence of fungi in the typical gut microbiome.
本研究分析野生和圈养苏门答腊猩猩的真菌群落。
采集9份野生猩猩粪便样本和9份圈养猩猩粪便样本，这些样本来自11至15岁健康状况良好的猩猩，并分为三个重复组。根据Qiime2和CCMetagen方法，使用Illumina平台分析ITS生物信息学。
野生苏门答腊猩猩的真菌群落包括53%的子囊菌门、38%的未培养真菌和4%的担子菌门。圈养猩猩的真菌群落为57%的子囊菌门、26%的未培养真菌和2%的担子菌门。在属水平上，野生猩猩中发现未培养的（31%）、（10%）、（3%）、（3%）、（2%）、（2%）和（2%）。圈养猩猩中最常见的属是（32%），其次是真菌属（11%）、（18%）、（2%）和（2%）。根据Chao1多样性指数以及香农和辛普森指数，野生和圈养的苏门答腊猩猩之间没有显著差异。
是野生苏门答腊猩猩所特有的，尽管在圈养猩猩中占主导地位。我们假设野生猩猩的肠道真菌群落与圈养猩猩相似。森林中丰富的食物来源并未导致典型肠道微生物群中真菌的流行。
