花生种子发育过程中的动态DNA甲基化修饰

Dynamic DNA methylation modification in peanut seed development.

作者信息

Li Zhongfeng, Liu Qian, Zhao Kai, Cao Di, Cao Zenghui, Zhao Kunkun, Ma Qian, Zhai Gaidan, Hu Sasa, Li Zhan, Wang Kuopeng, Gong Fangping, Ma Xingli, Zhang Xingguo, Ren Rui, Qiu Ding, Zhao Yu, Yin Dongmei

机构信息

College of Agronomy, Henan Agricultural University, Zhengzhou 450046, Henan Province, China.

National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China.

出版信息

iScience. 2023 Jun 7;26(7):107062. doi: 10.1016/j.isci.2023.107062. eCollection 2023 Jul 21.

DOI:10.1016/j.isci.2023.107062

PMID:37534185

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10391728/

Abstract

Cytosine methylation is an important epigenetic modification involved in regulation of plant development. However, the epigenetic mechanisms governing peanut seed development remain unclear. Herein, we generated DNA methylation profiles of developmental seeds of peanut H2014 and its smaller seed mutant H1314 at 15 and 60 days after pegging (DAP, S1, S4). Accompanying seed development, globally elevated methylation was observed in both lines. The mutant had a higher methylation level of 31.1% than wild type at S4, and 27.1-35.9% of the differentially methylated regions (DMRs) between the two lines were distributed in promoter or genic regions at both stages. Integrated methylome and transcriptome analysis revealed important methylation variations closely associated with seed development. Furthermore, some genes showed significantly negative correlation of expression with the methylation level within promoter or gene body. The results provide insights into the roles of DNA methylation in peanut seed development.

摘要

胞嘧啶甲基化是一种重要的表观遗传修饰，参与植物发育的调控。然而，控制花生种子发育的表观遗传机制仍不清楚。在此，我们生成了花生H2014及其较小种子突变体H1314在入土后15天和60天（DAP，S1，S4）发育种子的DNA甲基化图谱。随着种子发育，两个品系中均观察到整体甲基化水平升高。在S4阶段，突变体的甲基化水平比野生型高31.1%，两个品系之间27.1 - 35.9%的差异甲基化区域（DMRs）分布在两个阶段的启动子或基因区域。甲基化组和转录组的综合分析揭示了与种子发育密切相关的重要甲基化变异。此外，一些基因在启动子或基因体内的表达与甲基化水平呈显著负相关。这些结果为DNA甲基化在花生种子发育中的作用提供了见解。