Hayes D J, Challiss R A, Radda G K
Biochem J. 1986 Jun 1;236(2):469-73. doi: 10.1042/bj2360469.
A small animal model of arterial insufficiency has been used to investigate enzymic alterations in the gastrocnemius, plantaris and soleus muscles of the hypoperfused limb. At 7 days after induction of arterial insufficiency by unilateral femoral artery ligation, there were significant increases in the maximal activities of hexokinase, phosphorylase and 6-phosphofructokinase, whereas the activities of citrate synthase and 2-oxoglutarate dehydrogenase remained unchanged. Similar increases in hexokinase, phosphorylase and 6-phosphofructokinase were still apparent 8-10 weeks after unilateral artery ligation, although only hexokinase remained significantly higher than contralateral control values. No enhancement of oxidative enzyme activities was observed. The results are discussed in relation to the conflicting findings reported by other groups investigating enzymic adaptations in patients with arterial insufficiency.
一种小动物动脉供血不足模型已被用于研究灌注不足肢体的腓肠肌、跖肌和比目鱼肌中的酶变化。在通过单侧股动脉结扎诱导动脉供血不足7天后，己糖激酶、磷酸化酶和6-磷酸果糖激酶的最大活性显著增加，而柠檬酸合酶和2-氧代戊二酸脱氢酶的活性保持不变。单侧动脉结扎8 - 10周后，己糖激酶、磷酸化酶和6-磷酸果糖激酶仍有类似的增加，尽管只有己糖激酶仍显著高于对侧对照值。未观察到氧化酶活性增强。结合其他研究动脉供血不足患者酶适应性的小组报告的相互矛盾的结果对这些结果进行了讨论。
