Maduagwu E N, Umoh I B
Biochem Pharmacol. 1986 Sep 15;35(18):3003-6. doi: 10.1016/0006-2952(86)90378-3.
The biliary excretion of linamarin (2[beta-D-glucopyranosyloxy]isobutyronitrile) was studied in male albino Wistar rats injected i.p. with single doses of 300 mg linamarin/kg following cannulation of the bile duct in vivo; 24 hr faeces of uncannulated rats, similarly dosed, was examined for excretory products. Enzymatic and spectrophotometric analyses of the bile exudate showed that glucosidic cyanide (linamarin, and non-glucosidic cyanide were excreted; the elimination of both cyanide forms exhibits biphasic kinetics. Thiocyanate ion was undetectable. T.l.c. of the test bile followed by enzymatic and chemical investigation of the chromatogram confirmed the presence of unchanged linamarin, and four different u.v. fluorescent non-glucosidic cyanide metabolites. Neither linamarin nor cyanide ion was detectable in faeces of the uncannulated rats.
在体内胆管插管后，对雄性白化Wistar大鼠腹腔注射单剂量300mg亚麻苦苷/千克，研究了亚麻苦苷（2-[β-D-吡喃葡萄糖氧基]异丁腈）的胆汁排泄情况；对同样给药的未插管大鼠的24小时粪便进行了排泄产物检查。胆汁渗出液的酶促和分光光度分析表明，葡糖苷氰化物（亚麻苦苷）和非葡糖苷氰化物均被排泄；两种氰化物形式的消除均呈现双相动力学。未检测到硫氰酸根离子。对受试胆汁进行薄层层析，随后对色谱图进行酶促和化学研究，证实存在未变化的亚麻苦苷以及四种不同的紫外荧光非葡糖苷氰化物代谢产物。在未插管大鼠的粪便中未检测到亚麻苦苷和氰离子。
