Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) / Spanish National Research Council (CSIC) - University of Seville - University Pablo de Olavide, Sevilla, Spain.
PLoS Biol. 2023 Aug 3;21(8):e3002224. doi: 10.1371/journal.pbio.3002224. eCollection 2023 Aug.
Both the spindle microtubule-organizing centers and the nuclear pore complexes (NPCs) are convoluted structures where many signaling pathways converge to coordinate key events during cell division. Interestingly, despite their distinct molecular conformation and overall functions, these structures share common components and collaborate in the regulation of essential processes. We have established a new link between microtubule-organizing centers and nuclear pores in budding yeast by unveiling an interaction between the Bfa1/Bub2 complex, a mitotic exit inhibitor that localizes on the spindle pole bodies, and the Nup159 nucleoporin. Bfa1/Bub2 association with Nup159 is reduced in metaphase to not interfere with proper spindle positioning. However, their interaction is stimulated in anaphase and assists the Nup159-dependent autophagy pathway. The asymmetric localization of Bfa1/Bub2 during mitosis raises the possibility that its interaction with Nup159 could differentially promote Nup159-mediated autophagic processes, which might be relevant for the maintenance of the replicative lifespan.
纺锤体微管组织中心和核孔复合物（NPCs）都是卷曲结构，许多信号通路在那里汇聚，以协调细胞分裂过程中的关键事件。有趣的是，尽管它们具有不同的分子构象和总体功能，但这些结构具有共同的组成部分，并在调节基本过程中进行协作。我们通过揭示有丝分裂后期抑制剂 Bfa1/Bub2 复合物与核孔蛋白 Nup159 之间的相互作用，在芽殖酵母中建立了微管组织中心和核孔之间的新联系，该复合物定位于纺锤体极体上。Bfa1/Bub2 与 Nup159 的结合在中期减少，以不干扰纺锤体的正确定位。然而，它们的相互作用在后期被刺激，并协助 Nup159 依赖性自噬途径。Bfa1/Bub2 在有丝分裂期间的不对称定位提出了这样一种可能性，即其与 Nup159 的相互作用可能以不同的方式促进 Nup159 介导的自噬过程，这可能与维持复制寿命有关。
