Hussa R O, Pattillo R A, Ruckert A C, Rinke M L, Cortesi J P, Fein H G
Oncology. 1986;43(1):54-9. doi: 10.1159/000226105.
This study was aimed at optimizing large-scale roller bottle culture conditions for CaSki human cervical carcinoma cells, to produce ectopic hCG beta-like material in quantities sufficient for subsequent characterization studies. Several cell culture techniques contributed to the achievement of this goal: (1) use of serum-free culture medium; (2) use of intermittent recovery periods in presence of serum; and (3) ultrafiltration of the serum-free medium pool for initial concentration of 100-fold.
本研究旨在优化CaSki人宫颈癌细胞的大规模滚瓶培养条件，以产生足够数量的异位人绒毛膜促性腺激素β样物质，用于后续的特性研究。几种细胞培养技术有助于实现这一目标：（1）使用无血清培养基；（2）在有血清的情况下使用间歇恢复期；（3）对无血清培养基池进行超滤，初始浓缩100倍。
