Pattillo R A, Hussa R O, Story M T, Ruckert A C, Shalaby M R, Mattingly R F
Science. 1977 Jun 24;196(4297):1456-8. doi: 10.1126/science.867042.
Epidermoid cervical carcinoma cells (CaSki line) have been established in continuous culture. When leukocytes from cervical cancer patients were incubated with CaSki culture fluid concentrates, inhibition of leukocyte migration was observed in more than 70 percent of the patients tested. By contrast, significantly less inhibition was observed with normal donor leukocytes or leukocytes from patients with other types of cancer. These results were consistent with the expression of tumor-associated antigen by CaSki cells. Analysis of the serum from the donor of the cell line at the time of tumor biopsy, and of CaSki culture fluids, demonstrated the presence of the beta subunit of human chorionic gonadotropin.
表皮样子宫颈癌细胞（CaSki细胞系）已建立连续培养体系。当将子宫颈癌患者的白细胞与CaSki培养液浓缩物一起孵育时，在超过70%的受试患者中观察到白细胞迁移受到抑制。相比之下，正常供体白细胞或其他类型癌症患者的白细胞受到的抑制明显较少。这些结果与CaSki细胞表达肿瘤相关抗原一致。对肿瘤活检时该细胞系供体的血清以及CaSki培养液进行分析，证实存在人绒毛膜促性腺激素的β亚基。
