Liu Xiuxiu, Han Maoying, Weng Wendong, Li Yan, Pu Wenjuan, Liu Kuo, Li Xufeng, He Lingjuan, Sun Ruilin, Shen Ruling, He Yulong, Liang Dandan, Chen Yi-Han, Wang Qing-Dong, Tchorz Jan S, Zhou Bin
New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai, Shandong, China.
NPJ Regen Med. 2023 Aug 3;8(1):41. doi: 10.1038/s41536-023-00318-y.
A genetic system, ProTracer, has been recently developed to record cell proliferation in vivo. However, the ProTracer is initiated by an infrequently used recombinase Dre, which limits its broad application for functional studies employing floxed gene alleles. Here we generated Cre-activated functional ProTracer (fProTracer) mice, which enable simultaneous recording of cell proliferation and tissue-specific gene deletion, facilitating broad functional analysis of cell proliferation by any Cre driver.
一种名为ProTracer的遗传系统最近已被开发出来用于记录体内细胞增殖情况。然而，ProTracer是由一种不常用的重组酶Dre启动的，这限制了它在使用loxP侧翼基因等位基因进行功能研究中的广泛应用。在此，我们构建了Cre激活的功能性ProTracer（fProTracer）小鼠，其能够同时记录细胞增殖和组织特异性基因缺失情况，便于通过任何Cre驱动子对细胞增殖进行广泛的功能分析。
