Liu Rui, Chen Mengfei, Liu Boliang, Huang Kaiwei, Mao Zhenchuan, Li Huixia, Zhao Jianlong
College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu, China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2023 Jul 19;14:1217863. doi: 10.3389/fmicb.2023.1217863. eCollection 2023.
Root-knot nematode (RKN; spp.) is one of the most infamous soilborne plant diseases, causing severe crop losses every year. Effector proteins secreted by RKNs play crucial roles during plant-nematode interaction. However, less is known about whether RKN effector proteins can impact the rhizosphere microbial environment.
In this study, we investigated the rhizosphere microbiome community of (a plant immunity-modulating effector) transgenic with or without nematode infection using the Illumina high-throughput sequencing analysis.
The results showed that the bacterial species richness index increased, while the fungi species richness index decreased in -infected transgenic plants. The relative abundance of genera such as , and was reduced in transgenic plants compared to wild type, but was significantly increased after inoculation with . The Cluster of Orthologous Genes (COG) function classification analysis revealed a decrease in the relative abundance of defense mechanisms, secondary metabolite biosynthesis, transport, and nematode infection catabolism-related functions in lines compared to the wild type. These differences may be the reason for the increased susceptibility of transgenic to nematode infection. Our results provide a new insight into RKN effector proteins and their association with the microbial community, host, and plant pathogens, which will lead to the exploration of new innovative ideas for future biological control of RKNs.
根结线虫（RKN； 属）是最臭名昭著的土传植物病害之一，每年都会造成严重的作物损失。根结线虫分泌的效应蛋白在植物与线虫的相互作用中起着关键作用。然而，关于根结线虫效应蛋白是否会影响根际微生物环境，我们所知甚少。
在本研究中，我们使用Illumina高通量测序分析，研究了感染或未感染线虫的（一种植物免疫调节效应蛋白）转基因 的根际微生物群落。
结果表明，在感染线虫的转基因 植物中，细菌物种丰富度指数增加，而真菌物种丰富度指数降低。与野生型相比，转基因 植物中 、 和 等属的相对丰度降低，但接种线虫后显著增加。直系同源基因（COG）功能分类分析表明，与野生型相比， 系中防御机制、次生代谢物生物合成、转运以及与线虫感染分解代谢相关功能的相对丰度降低。这些差异可能是转基因 对线虫感染易感性增加的原因。我们的研究结果为根结线虫效应蛋白及其与微生物群落、宿主和植物病原体的关联提供了新的见解，这将为未来根结线虫生物防治探索新的创新思路。
