Lu Cuihua, Li Erfeng, Liu Rui, Chang Nv, Lai Yuqing, Wu Yue, Wu Weilong, Chen Zhukan, Ling Jian, Zhao Jianlong, Mao Zhenchuan
College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin, China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2025 Apr 17;16:1546583. doi: 10.3389/fmicb.2025.1546583. eCollection 2025.
Root-knot nematodes (RKNs), belonging to the genus Meloidogyne, are plant parasitic nematodes with a broad host range, causing substantial economic losses annually. The selection and utilization of novel biological control resources are crucial for managing RKNs diseases.
This study isolated Cq-C08 from rhizosphere soil, which the efficacy of Cq-C08 against was investigated through laboratory experiments, pot and plot trials, and analysis of the transcriptomic data from cucumber roots treated with Cq-C08.
This study isolated Cq-C08 from rhizosphere soil, and a series of experiments confirmed that the fermentation broth had a lethal rate of 100% against J2s within 12 h and exhibited a significant repellent effect on the nematodes. In pot and plot tests, the strain Cq-C08 achieved a control effect over 50% against and significantly promoted cucumber (, Zhongnong No. 6, China) growth. Inoculation experiments confirmed that the Cq-C08 strain could activate key immune signaling pathways of salicylic acid (SA) and jasmonic acid (JA). Split-root tests showed significant induced resistance of cucumber to by 32.3%. Comparative transcriptome analysis confirmed that strain Cq-C08 could regulate the host's basal immune response and oxidative burst response through SA, JA, and ethylene (ET) signaling pathways, and alter secondary metabolism, activating the synthesis of cucurbitacin and auxins, which promotes plant immune regulation and growth. These results prove that Cq-C08 has high control effects against and the potential to be developed into a biological control product against root-knot nematodes.
根结线虫（RKNs）属于根结线虫属，是一类寄主范围广泛的植物寄生线虫，每年造成巨大的经济损失。新型生物防治资源的筛选和利用对于管理根结线虫病害至关重要。
本研究从根际土壤中分离出Cq-C08，通过实验室实验、盆栽和田间试验以及对用Cq-C08处理的黄瓜根的转录组数据分析，研究了Cq-C08对[未提及具体对象]的防治效果。
本研究从根际土壤中分离出Cq-C08，一系列实验证实，发酵液在12小时内对[未提及具体对象]的J2s致死率达100%，并对线虫表现出显著的驱避作用。在盆栽和田间试验中，Cq-C08菌株对[未提及具体对象]的防治效果超过50%，并显著促进了黄瓜（[未提及具体品种信息]，中国中农6号）的生长。接种实验证实，Cq-C08菌株可激活水杨酸（SA）和茉莉酸（JA）的关键免疫信号通路。分根试验表明，黄瓜对[未提及具体对象]的诱导抗性显著提高了32.3%。比较转录组分析证实，Cq-C08菌株可通过SA、JA和乙烯（ET）信号通路调节寄主的基础免疫反应和氧化爆发反应，并改变次生代谢，激活葫芦素和生长素的合成，从而促进植物的免疫调节和生长。这些结果证明，Cq-C08对[未提及具体对象]具有较高的防治效果，具有开发成防治根结线虫生物防治产品的潜力。
