Dataset on the single nucleotide variation in diversity panel of 500 lettuce accessions genotyped with tunable genotyping-by-sequencing (tGBS) method.

Lettuce ( L.) is an important leafy vegetable cultivated in moderate climates around the world. Based on phenotypic characteristics, such as formation and size of 'heads' (leaves arranged in a dense rosette), size, and texture of leaves, length of stems, and size and composition of seeds, lettuce cultivars can be classified into several distinct horticultural types (Batavia, butterhead, iceberg, Latin, leaf, oilseed, romaine, and stem). , a wild progenitor of cultivated lettuce, is frequently utilized in breeding programs to introgress desirable genes and alleles (e.g., resistance to diseases) into lettuce gene pool. A diversity panel of ∼500 lettuce accessions was genotyped with tunable genotyping-by-sequencing (tGBS) method to identify single nucleotide polymorphism (SNP) sites. 115,261 SNPs were positioned on lettuce genome using the reference genome of cultivar Salinas. The described diversity panel together with the set of SNP markers can be used for mapping quantitative trait loci (QTL) and to develop marker assays for marker assisted selection (MAS). Identified SNP sites can also be used to identify F hybrids, genotype gene bank collections, and in other areas of lettuce genetics and breeding.