Dohnal J C, Bowie L J, Burstein H J
Clin Chem. 1986 Mar;32(3):425-8.
To study the effect of fatty acid chain saturation on the fluorescence polarization assay as a measure of fetal lung maturity, we used purified phospholipids isolated from human amniotic fluid and various commercial phospholipids. We found that the fluorescence polarization value decreased as the concentration of unsaturated fatty acids increased. In contrast, the lecithin/sphingomyelin ratio increases with increasing amounts of saturated lecithin, produced as the fetal lung matures. Since only saturated lecithins are surface active, the two indices of fetal respiratory status must reflect different properties of lung surfactant.
为了研究脂肪酸链饱和度对作为胎儿肺成熟度指标的荧光偏振测定法的影响，我们使用了从人羊水分离出的纯化磷脂以及各种市售磷脂。我们发现，随着不饱和脂肪酸浓度的增加，荧光偏振值降低。相比之下，随着胎儿肺成熟产生的饱和卵磷脂含量增加，卵磷脂/鞘磷脂比值升高。由于只有饱和卵磷脂具有表面活性，胎儿呼吸状态的这两个指标必定反映了肺表面活性剂的不同特性。
