Zhang Wenhui, Zhang Xingping, Yan Deqi, Wang Guanying, Wang Qingquan, Ren Xiaojuan, Liu Tao
Xinjiang Medical University, The Fourth Clinical College of Xinjiang Medical University, China.
College of Traditional Chinese Medicine, Guizhou University of Traditional Chinese Medicine, China.
Heliyon. 2023 Jul 17;9(7):e18338. doi: 10.1016/j.heliyon.2023.e18338. eCollection 2023 Jul.
It is well known that stressful situation is one of the important factors causing insomnia, however, the underlying mechanism is still elusive. Therefore, the establishment of a suitable animal model of stress insomnia will be of great help to solve this problem. In this study, by combining with chronic unpredictable stress (multitude of stressors) and sleep deprivation, we attempted to establish a rat model of stress insomnia. It was observed that rats with stress insomnia showed significant weight loss, and less sleep quality in pentobarbital sodium induced sleep test and electroencephalogram detection. Moreover, rats with stress insomnia showed greater depression and anxiety detected by forced swimming, sucrose preference test and open field. Since oxidative stress has been reported to be involved in insomnia, we further evaluated the production of oxidative stress and found that the levels of lipid peroxidation product malondialdehyde (MDA) in liver, serum total bilirubin and urine biopyrrin were all significantly increased in rats with stress insomnia. In addition, we also found that the memory of these rats with stress insomnia was also obviously reduced in water maze. Taken together, these results demonstrate that the emotional behaviors, memory, oxidative and metabolism of the rats were all significantly changed after modeling, indicating a rat model of stress insomnia was successful establishment, and this animal model will provide basis to further explore the underlying mechanism of chronic stress in insomnia.
众所周知，应激状态是导致失眠的重要因素之一，然而其潜在机制仍不清楚。因此，建立合适的应激性失眠动物模型将有助于解决这一问题。在本研究中，通过结合慢性不可预测应激（多种应激源）和睡眠剥夺，我们试图建立应激性失眠大鼠模型。观察到应激性失眠大鼠体重显著减轻，在戊巴比妥钠诱导睡眠试验和脑电图检测中睡眠质量较差。此外，通过强迫游泳、蔗糖偏好试验和旷场试验检测发现，应激性失眠大鼠表现出更严重的抑郁和焦虑。由于氧化应激已被报道与失眠有关，我们进一步评估了氧化应激的产生，发现应激性失眠大鼠肝脏中脂质过氧化产物丙二醛（MDA）水平、血清总胆红素和尿双吡咯啉均显著升高。此外，我们还发现这些应激性失眠大鼠在水迷宫中的记忆也明显减退。综上所述，这些结果表明建模后大鼠的情绪行为、记忆、氧化和代谢均发生了显著变化，表明成功建立了应激性失眠大鼠模型，该动物模型将为进一步探索慢性应激导致失眠的潜在机制提供依据。
