Kanoh M, Utsumi S, Hino T
Eur J Immunol. 1986 Jan;16(1):63-8. doi: 10.1002/eji.1830160113.
Administration of 2,4,6-trinitrophenylated E. coli lipopolysaccharide (TNP-LPS) complexed with mouse IgG antibody to TNP specifically gave rise to a marked production of rheumatoid-like factors (RF) in the recipient mice, in contrast to the low and nonspecific RF production via polyclonal B cell activation by the same dosage of LPS or TNP-LPS alone. The RF activity induced by the LPS immune complexes was associated with both IgG and IgM and directed primarily to the C gamma 2 region as judged by the heterophilic reactivity toward fragments of rabbit IgG. The results suggest that antibody molecules attached to LPS constitute novel epitopic groups on the mitogenic carrier and stimulate B cells in a specific manner to induce the autoantibodies.
将与小鼠抗三硝基苯（TNP）IgG抗体复合的2,4,6-三硝基苯化大肠杆菌脂多糖（TNP-LPS）给予受体小鼠，会使其显著产生类风湿样因子（RF），这与相同剂量的LPS或单独的TNP-LPS通过多克隆B细胞激活引起的低水平非特异性RF产生形成对比。LPS免疫复合物诱导的RF活性与IgG和IgM均有关，并且通过对兔IgG片段的嗜异性反应判断，主要针对Cγ2区域。结果表明，附着于LPS的抗体分子在促有丝分裂载体上构成了新的表位基团，并以特定方式刺激B细胞以诱导自身抗体。
