Van Snick J, Coulie P
Eur J Immunol. 1983 Nov;13(11):890-4. doi: 10.1002/eji.1830131106.
The involvement of rheumatoid factor (RF)-secreting cells in normal immune responses was examined by screening hybridomas derived from 129/Sv mice during primary and secondary immune responses against foreign proteins. No RF-secreting cells were detected during primary responses, but up to 10% of the total number of clones obtained during secondary responses produced IgM with anti-IgG activity. Like typical mouse RF, these anti-IgG autoantibodies had a strict isotypic specificity, mostly for IgG1, and a much stronger avidity for immune complexes than for native IgG. The selective activation of IgG1-specific RF during these secondary immune reactions was not due to fortuitous antigenic similarities between mouse IgG1 and the antigens used for immunization, nor did it result from the use of adjuvants for priming, or from contamination of antigen preparations with lipopolysaccharide. It is therefore concluded that, in the 129/Sv mouse, RF production during secondary immune responses is a physiological phenomenon.
通过筛选在针对外源蛋白的初次和二次免疫反应期间源自129/Sv小鼠的杂交瘤，研究了分泌类风湿因子（RF）的细胞在正常免疫反应中的参与情况。在初次反应期间未检测到分泌RF的细胞，但在二次反应期间获得的克隆总数中，高达10%产生具有抗IgG活性的IgM。与典型的小鼠RF一样，这些抗IgG自身抗体具有严格的同种型特异性，主要针对IgG1，并且对免疫复合物的亲和力比对天然IgG强得多。在这些二次免疫反应期间IgG1特异性RF的选择性激活不是由于小鼠IgG1与用于免疫的抗原之间偶然的抗原相似性，也不是由于使用佐剂进行启动，或抗原制剂被脂多糖污染所致。因此得出结论，在129/Sv小鼠中，二次免疫反应期间RF的产生是一种生理现象。
