Ramos-Niembro F, Fournié G, Lambert P H
Kidney Int Suppl. 1982 May;11:S29-38.
We investigated the ability of various polyclonal B-cell activators (PBA) to induce immunoglobulin synthesis, circulating immune complexes, and rheumatoid-factor-like autoantibodies. We found that, following the injection of a PBA--bacterial lipopolysaccharide, dextran sulfate, polyriboinosinic-polyribocytidilic acid, or purified protein derivative of tubercle bacteria--a transitory formation of circulating immune complexes occurred simultaneously with an increase in immunoglobulin production. The presence of circulating immune complexes after PBA administration was documented by the 125I-Clq-binding assay and the conglutinin-binding assay, and a partial characterization of this material was achieved. Although the kinetic properties, size, and composition of the immune complexes tested varied with the PBA used, the complexes detected in each group were inactivated by mild reduction and alkylation with 2-mercaptoethanol and were unaffected by DNase treatment. In the mice injected with bacterial lipopolysaccharide, the induction of circulating immune complexes correlated significantly with the presence of rheumatoid-factor-like antibodies, suggesting that this autoantibody may be present within the detected immune complexes. In tissues, glomerular deposits of IgM, IgG, and C3 were observed in a pattern compatible with the deposition of immune complexes. These deposits were progressively associated with marked glomerular abnormalities in mice chronically injected with LPS during 1 year. These data suggest that the induction of polyclonal antibody synthesis, which occurs in a variety of infectious or autoimmune diseases, may be responsible for the high incidence and persistence of immune complexes in these diseases. Such complexes would involve primarily autoantigens and corresponding autoantibodies, such as rheumatoid factor IgG complexes, without the participation of any specific bacterial, viral, or parasitic antigen.
我们研究了各种多克隆B细胞激活剂（PBA）诱导免疫球蛋白合成、循环免疫复合物和类风湿因子样自身抗体的能力。我们发现，在注射一种PBA——细菌脂多糖、硫酸葡聚糖、聚肌苷酸-聚胞苷酸或结核杆菌纯化蛋白衍生物后——循环免疫复合物的短暂形成与免疫球蛋白产生的增加同时发生。通过125I-Clq结合试验和胶固素结合试验记录了PBA给药后循环免疫复合物的存在，并对该物质进行了部分特性鉴定。尽管所测试的免疫复合物的动力学特性、大小和组成因所用的PBA而异，但每组中检测到的复合物经2-巯基乙醇轻度还原和烷基化后失活，且不受DNase处理的影响。在注射细菌脂多糖的小鼠中，循环免疫复合物的诱导与类风湿因子样抗体的存在显著相关，这表明这种自身抗体可能存在于检测到的免疫复合物中。在组织中，观察到IgM、IgG和C3的肾小球沉积物，其模式与免疫复合物的沉积一致。在1年期间长期注射LPS的小鼠中，这些沉积物逐渐与明显的肾小球异常相关。这些数据表明,在多种感染性或自身免疫性疾病中发生的多克隆抗体合成的诱导，可能是这些疾病中免疫复合物高发生率和持续存在的原因。此类复合物主要涉及自身抗原和相应的自身抗体，如类风湿因子IgG复合物，而无需任何特定细菌、病毒或寄生虫抗原的参与。
