Laboratory of Marine Environmental Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
Depertment of Marine and Earth Sciences, Marine Works Japan Ltd., 3-54-1 Oppamahigashi, Yokosuka 237-0063, Japan.
Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.006014.
A novel mesophilic, obligately anaerobic, facultatively sulphur-reducing bacterium, designated strain IC12, was isolated from a deep-sea hydrothermal field in the Mid-Okinawa Trough, Japan. The cells were Gram-negative, motile, short rods with a single polar flagellum. The ranges and optima of the growth temperature, NaCl concentration and pH of strain IC12 were 15-40 °C (optimum, 30-35 °C), 10-60 g l (optimum, 20-30 g l) and pH 4.9-6.7 (optimum, pH 5.8), respectively. Yeast extract was utilized as a sole carbon and energy source for fermentative growth. Major fatty acids of strain IC12 were C, C and C ω7. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IC12 was affiliated to the phylum and was most closely related to VenChi2 (86.5 % sequence similarity). Strain IC12 contained a chromosome of 2.43 Mbp and a large plasmid of 0.30 Mbp. The G+C content of the genomic DNA was 26.4 mol%. The maximum values for average nucleotide identity and DNA-DNA hybridization between strain IC12 and related strains of the phylum were 71.4 and 26.4 %, respectively. Phylogenomic, physiological and chemotaxonomic analyses indicate that strain IC12 represents a novel genus and species within the phylum , for which the name gen. nov., sp. nov. is proposed, with strain IC12 (= DSM 112164=JCM 39166) as the type strain. We also propose the family fam. nov. to accommodate this novel genus.
一株新型嗜中温、严格厌氧、兼性硫酸盐还原菌，命名为 IC12 菌株，从日本中太平洋海槽深海热液场中分离得到。细胞呈革兰氏阴性，可运动，短杆状，有单个极生鞭毛。IC12 菌株的生长温度、NaCl 浓度和 pH 值范围及最适范围分别为 15-40°C（最适 30-35°C）、10-60 g l（最适 20-30 g l）和 pH4.9-6.7（最适 pH5.8）。酵母提取物可作为发酵生长的唯一碳源和能源。IC12 菌株的主要脂肪酸为 C、C 和 Cω7。基于 16S rRNA 基因序列的系统发育分析结果表明，菌株 IC12 属于门，与 VenChi2 最为密切相关（86.5%序列相似性）。IC12 菌株含有一条 2.43 Mbp 的染色体和一条 0.30 Mbp 的大型质粒。基因组 DNA 的 G+C 含量为 26.4 mol%。菌株 IC12 与门内相关菌株的平均核苷酸同一性和 DNA-DNA 杂交的最大值分别为 71.4%和 26.4%。系统发育基因组学、生理学和化学分类学分析表明，菌株 IC12 代表了一个新的属和种，属于门，命名为 ，新属，种。提议，IC12 株（=DSM 112164=JCM 39166）为模式菌株。我们还提议建立一个新的科 ，以容纳这个新的属。
