Tamazawa Satoshi, Mayumi Daisuke, Mochimaru Hanako, Sakata Susumu, Maeda Haruo, Wakayama Tatsuki, Ikarashi Masayuki, Kamagata Yoichi, Tamaki Hideyuki
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki, Japan.
Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki, Japan.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3982-3986. doi: 10.1099/ijsem.0.002234. Epub 2017 Sep 12.
A novel thermophilic, anaerobic, chemoheterotrophic, acetate-oxidizing and iron(III)-, manganese(IV)-, nitrate- and sulfate-reducing bacterium, designated strain ANA, was isolated from a deep subsurface oil field in Japan (Yabase oil field, Akita Pref.). Cells of strain ANA were Gram-stain-negative, non-motile, non-spore forming and slightly curved or twisted rods (1.5-5.0 µm long and 0.6-0.7 µm wide). The isolate grew at 25-60 °C (optimum 55 °C) and pH 6.0-8.0 (optimum pH 7.0). The isolate was capable of reducing iron(III), manganese(IV), nitrate and sulfate as an electron acceptor. The isolate utilized a limited range of electron donors such as acetate, lactate, pyruvate and yeast extract for iron reduction. Strain ANA also used pyruvate, fumarate, succinate, malate, yeast extract and peptone for fermentative growth. The major respiratory quinones were menaquinone-7(H8) and menaquinone-8. The strain contained C18 : 0, iso-C18 : 0 and C16 : 0 as the major cellular fatty acids. The G+C content of the genomic DNA was 34.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ANA was closely related to Calditerrivibrio nitroreducens in the phylum Deferribacteres with low sequence similarities (89.5 %), and formed a distinct clade within the family Deferribacteraceae. In addition, the isolate is the first sulfate-reducing member of the phylum Deferribacteres. Based on phenotypic, chemotaxonomic and phylogenetic properties, a novel genus and species, Petrothermobacter organivorans gen. nov., sp. nov., is proposed for the isolate (type strain=ANA= NBRC 112621=DSM 105015).
从日本一个深层地下油田（秋田县矢部油田）分离出一株新型嗜热、厌氧、化能异养、能氧化乙酸并还原铁（III）、锰（IV）、硝酸盐和硫酸盐的细菌，命名为菌株ANA。菌株ANA的细胞革兰氏染色阴性，不运动，不产芽孢，为稍弯曲或扭曲的杆菌（长1.5 - 5.0 µm，宽0.6 - 0.7 µm）。该分离株在25 - 60 °C（最适温度55 °C）和pH 6.0 - 8.0（最适pH 7.0）条件下生长。该分离株能够以铁（III）、锰（IV）、硝酸盐和硫酸盐作为电子受体进行还原。该分离株利用有限种类的电子供体如乙酸盐、乳酸盐、丙酮酸盐和酵母提取物进行铁还原。菌株ANA还利用丙酮酸盐、富马酸盐、琥珀酸盐、苹果酸盐、酵母提取物和蛋白胨进行发酵生长。主要呼吸醌为甲基萘醌-7（H8）和甲基萘醌-8。该菌株含有C18 : 0、异-C18 : 0和C16 : 0作为主要细胞脂肪酸。基因组DNA的G + C含量为34.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株ANA与脱铁杆菌门中的硝基还原嗜热栖泥菌密切相关，但序列相似性较低（89.5 %），并在脱铁杆菌科内形成一个独特的分支。此外，该分离株是脱铁杆菌门中首个能还原硫酸盐的成员。基于表型、化学分类和系统发育特性，为该分离株（模式菌株 = ANA = NBRC 112621 = DSM 105015）提出一个新属和新种，即有机嗜热石油杆菌属新属，有机嗜热石油杆菌新种。
