Song Rongqun, Wang Zhenlu, Lin Shaoqing, Guo Xingchen, Wang Yizhou, Zhang Lin, Ye Huan, Shao Jian
Laboratory of Fishery Resources and Environmental Protection, College of Animal Science, Guizhou University, Guiyang 550025, China.
The Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China.
Animals (Basel). 2024 Oct 21;14(20):3042. doi: 10.3390/ani14203042.
This work explores the digestive system characteristics of during early developmental stages and aims to solve the problem of high lethality of fry during the transgression period, which is crucial for the artificial propagation and population conservation of endangered fishes. This study was carried out on intestinal tissue, digestive enzymes, and antioxidant enzyme activities in the early development stage of . Ten random samples during endogenous nutrition (7, 10, and 11 days after hatching), mixed nutrition (13 and 19 DAH), and exogenous nutrition (31, 33, 39, 45, and 73 DAH) were collected by histological and biochemical analysis methods. The results showed that the intestine of already has four layers initially at 7 DAH, and the intestinal gland tissue is evident at 73 DAH. The contents of total protein (TP) and the activities of lipase (LPS) and trypsin (TPS) were maximal at 39 DAH, and the activities were 3.20 ± 0.26 mg/mL, 2.52 ± 0.69 U/g, and 2717.45 ± 295.26 U/mg, respectively. Catalase (CAT) and glutathione peroxidase (GSH-PX) activities both showed the lowest values at 39 DAH, which were 0.57 ± 0.11 U/mg and 3.35 ± 0.94 U/mg, respectively. The activity of amylase (AMS) and the content of malonaldehyde (MDA) increased, and the highest values were reached at 45 DAH (1.32 ± 0.41 U/mg) and 73 DAH (1.29 ± 0.43 nmoL/mg), respectively. Superoxide dismutase (SOD) and GSH-PX activities both showed a peak value at 7 DAH (126.58 ± 20.13 U/mg and 6.47 ± 1.86 U/mg). Overall, the changes in intestinal tissue, digestive enzymes, and antioxidant enzyme activities at 39 DAH of are inseparable from different vegetative stages during the developmental period, and these results can provide a reference for the proliferation and cultivation of resources.
本研究探讨了[鱼类名称]早期发育阶段的消化系统特征，旨在解决幼鱼在变态期高致死率的问题，这对濒危鱼类的人工繁殖和种群保护至关重要。本研究针对[鱼类名称]早期发育阶段的肠道组织、消化酶和抗氧化酶活性展开。通过组织学和生化分析方法，采集了内源性营养阶段（孵化后7、10和11天）、混合营养阶段（孵化后13和19天）以及外源性营养阶段（孵化后31、33、39、45和73天）的10个随机样本。结果表明，[鱼类名称]的肠道在孵化后7天最初就已具有四层结构，肠道腺组织在孵化后73天明显可见。总蛋白（TP）含量、脂肪酶（LPS）和胰蛋白酶（TPS）活性在孵化后39天达到最大值，分别为3.20±0.26mg/mL、2.52±0.69U/g和2717.45±295.26U/mg。过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-PX）活性在孵化后39天均显示出最低值，分别为0.57±0.11U/mg和3.35±0.94U/mg。淀粉酶（AMS）活性和丙二醛（MDA）含量增加，分别在孵化后45天（1.32±0.41U/mg）和73天（1.29±0.43nmoL/mg）达到最高值。超氧化物歧化酶（SOD）和GSH-PX活性在孵化后7天均显示出峰值（126.58±20.13U/mg和6.47±1.86U/mg）。总体而言，[鱼类名称]在孵化后39天肠道组织、消化酶和抗氧化酶活性的变化与发育期间不同营养阶段密不可分，这些结果可为[鱼类名称]资源的增殖和培育提供参考。
