Key Laboratory of Resource Utilization of Agricultural Solid Waste in Gansu Province, Tianshui Normal University, South Xihe Road, Qinzhou, Tianshui 741000, Gansu, PR China.
Key Laboratory of Resource Utilization of Agricultural Solid Waste in Gansu Province, Tianshui Normal University, South Xihe Road, Qinzhou, Tianshui 741000, Gansu, PR China.
Ecotoxicol Environ Saf. 2023 Nov 1;266:115607. doi: 10.1016/j.ecoenv.2023.115607. Epub 2023 Oct 19.
Tsinling lenok trout (Brachymystax lenok tsinlingensis Li) is a species of cold-water salmon that faces serious challenges due to global warming. High temperature stress has been found to damage the gut integrity of cold-water fish, impacting their growth and immunity. However, limited research exists on the causal relationship between gut microbial disturbance and metabolic dysfunction in cold-water fish induced by high temperature stress. To address this gap, we conducted a study to investigate the effects of high temperature stress (24 °C) on the gut tissue structure, antioxidant capacity, gut microorganisms, and metabolome reactions of tsinling lenok trout. Our analysis using 16 S rDNA gene sequencing revealed significant changes in the gut microbial composition and metabolic profile. Specifically, the abundance of Firmicutes and Gemmatimonadetes decreased significantly with increasing temperature, while the abundance of Bacteroidetes increased significantly. Metabolic analysis revealed a significant decrease in the abundance of glutathione, which is synthesized from glutamate and glycine, under high temperature stress. Additionally, there was a notable reduction in the levels of adenosine, inosine, xanthine, guanosine, and deoxyguanosine, which are essential for DNA/RNA synthesis. Conversely, there was a significant increase in the abundance of D-glucose 6 P. Furthermore, high temperature stress adversely affects intestinal structure and barrier function. Our findings provide valuable insights into the mechanism of high temperature stress in cold-water fish and serve as a foundation for future research aimed at mitigating the decline in production performance caused by such stress.
秦岭细鳞鲑（Brachymystax lenok tsinlingensis Li）是一种冷水鲑鱼，由于全球变暖，面临着严重的挑战。高温应激已被发现会破坏冷水鱼的肠道完整性，影响其生长和免疫力。然而，关于高温应激引起的冷水鱼肠道微生物扰动和代谢功能障碍之间的因果关系的研究有限。为了解决这一差距，我们进行了一项研究，调查了高温应激（24°C）对秦岭细鳞鲑肠道组织结构、抗氧化能力、肠道微生物和代谢组反应的影响。我们使用 16S rDNA 基因测序的分析显示，肠道微生物组成和代谢谱发生了显著变化。具体来说，厚壁菌门和芽单胞菌门的丰度随着温度的升高显著下降，而拟杆菌门的丰度显著增加。代谢分析显示，在高温应激下，合成自谷氨酸和甘氨酸的谷胱甘肽的丰度显著降低。此外，腺嘌呤、肌苷、黄嘌呤、鸟嘌呤和脱氧鸟嘌呤的水平显著降低，这些物质是 DNA/RNA 合成所必需的。相反，D-葡萄糖 6-磷酸的丰度显著增加。此外，高温应激会对肠道结构和屏障功能产生不利影响。我们的研究结果为冷水鱼高温应激的机制提供了有价值的见解，并为未来旨在减轻此类应激引起的生产性能下降的研究奠定了基础。
