Department of Bioengineering, Faculty of Engineering and Architecture , Konya Food and Agriculture University , 42080 Konya , Turkey.
Research and Development Center for Diagnostic Kits (KIT-ARGEM) , Konya Food and Agriculture University , 42080 Konya , Turkey.
Anal Chem. 2018 Mar 20;90(6):4221-4225. doi: 10.1021/acs.analchem.8b00576. Epub 2018 Mar 7.
A novel ratiometric colorimetric and fluorescent dual probe based on Eriochrome Black T (EBT)-Eu complex was designed to detect dipicolinic acid (DPA), a major constituent of bacterial spores, with high sensitivity and selectivity. UV-vis titration experiments demonstrated that EBT and Eu ions formed a 1:1 coordination pair in water. In the presence of Eu ions, the blue solution of EBT changed to magenta, however, upon the addition of DPA, the magenta color changed to blue immediately and characteristic fluorescence emission from DPA-Eu complex was observed. In addition, the sensitivity of the system was further evaluated on Geobacillus stearothermophilus spores and as low as 2.5 × 10 spores were detected.
基于变色酸黑 T（EBT）-Eu 配合物的比率型比色和荧光双探针用于检测细菌芽孢的主要成分二吡啶羧酸（DPA），具有高灵敏度和选择性。紫外可见滴定实验表明，EBT 和 Eu 离子在水中形成 1:1 的配位对。在 Eu 离子存在下，EBT 的蓝色溶液变为洋红色，然而，当加入 DPA 时，洋红色立即变为蓝色，并观察到 DPA-Eu 配合物的特征荧光发射。此外，还进一步评估了该体系对嗜热脂肪芽孢杆菌芽孢的灵敏度，检测下限低至 2.5×10 个芽孢。
